Peripheral noxious stimulation induces phosphorylation of the NMDA receptor NR1 subunit at the PKC-dependent site, serine-896, in spinal cord dorsal horn neurons.

The European Journal of Neuroscience
Gary J BrennerClifford J Woolf

Abstract

The N-methyl-D-aspartate receptor (NMDAR) contributes to central sensitization in the spinal cord and the generation of pain hypersensitivity. NMDAR function is modulated by post-translational modifications including phosphorylation, and this is proposed to underlie its involvement in the production of pain hypersensitivity in the spinal cord. We now show that a noxious heat stimulus applied to the rat hindpaw induces phosphorylation of the NMDAR NR1 subunit at a protein kinase C (PKC)-dependent site, serine-896, in superficial dorsal horn neurons. Phosphorylation of NR1 serine-896 is essentially absent in the superficial dorsal horn laminae of naïve rats, but there is rapid (< 2 min) induction following a noxious but not innocuous heat stimulus. The number of pNR1-immunoreactive neuronal profiles in the superficial dorsal horn peaks 30 min after noxious heat stimulation and persists for up to 1 h. pNR1serine896 induction occurs in the endoplasmic reticulum, suggesting that it contributes to trafficking of the receptor from intracellular stores to the membrane. The phosphorylation of the subunit is attenuated by intrathecal injection of the NMDAR antagonist, MK801, suggesting that the NMDAR is involved via a feed-forward mechan...Continue Reading

References

Jan 1, 1992·Progress in Neurobiology·R D TreedeJ N Campbell
Aug 25, 1995·The Journal of Biological Chemistry·L F Lau, R L Huganir
Oct 1, 1995·Neuropharmacology·H Mori, M Mishina
Aug 15, 1997·The Journal of Biological Chemistry·C HisatsuneT Yamamoto
Mar 3, 1998·Progress in Neurobiology·G Baranauskas, A Nistri
Apr 29, 1998·Progress in Neurobiology·S OzawaK Tsuzuki
Aug 28, 1999·Progress in Neurobiology·T Yamakura, K Shimoji
Jun 10, 2000·Science·C J Woolf, M W Salter
Mar 29, 2001·Nature Neuroscience·J Y LanR S Zukin
Jul 4, 2002·The Journal of Biological Chemistry·Zoltan OlahMichael J Iadarola
Aug 6, 2002·Neuron·Andres Barria, Roberto Malinow
Oct 24, 2002·Trends in Neurosciences·Reed C Carroll, R Suzanne Zukin

❮ Previous
Next ❯

Citations

Oct 31, 2009·Molecular Neurobiology·Max Larsson
Oct 1, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Thierry Bordet, Rebecca M Pruss
Jun 17, 2006·Annual Review of Neuroscience·Sophie Pezet, Stephen B McMahon
Jun 1, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wei GuoKe Ren
Dec 16, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chi H E MaTarek A Samad
Jan 24, 2006·Journal of the American Veterinary Medical Association·Antonio PozziFrancesca Traverso
Jun 4, 2015·Expert Opinion on Drug Discovery·Mellar P Davis
Jan 7, 2015·Molecular Pain·Manouela V ValtchevaRobert W Gereau
Jul 3, 2013·The Journal of Pain : Official Journal of the American Pain Society·Ricardo KusudaGuilherme Lucas
Feb 1, 2011·Free Radical Biology & Medicine·Daniela SalveminiWilliam L Neumann
Jul 14, 2010·The Journal of Pain : Official Journal of the American Pain Society·Robert M CaudleJohn K Neubert
Feb 3, 2009·The Journal of Pain : Official Journal of the American Pain Society·Sandra M GarrawayCharles E Inturrisi
Jan 27, 2009·Brain Research Reviews·Ke Ren, Richard Torres
Dec 9, 2008·Experimental Neurology·Emily L HoschouerLyn B Jakeman
Apr 17, 2007·The Journal of Pain : Official Journal of the American Pain Society·Gretchen J SummerJon D Levine
Feb 18, 2009·Headache·Montree ManeepakAnan Srikiatkhachorn
Mar 17, 2006·Journal of Neurochemistry·V S SeyboldP G Mermelstein
Feb 19, 2008·The European Journal of Neuroscience·Alin L AkopiansPatricia E Phelps
Sep 1, 2006·Neuroscience Letters·QiQi ZhouG Nicholas Verne
Jan 18, 2006·Molecular and Cellular Neurosciences·Jing ZhaoUNKNOWN London Pain Consortium
Dec 28, 2011·Biochimica Et Biophysica Acta·Kali JanesDaniela Salvemini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.