Peripheral re-localization of constitutive heterochromatin advances its replication timing and impairs maintenance of silencing marks

Nucleic Acids Research
Kathrin S HeinzM C Cardoso

Abstract

The replication of the genome is a highly organized process, both spatially and temporally. Although a lot is known on the composition of the basic replication machinery, how its activity is regulated is mostly unknown. Several chromatin properties have been proposed as regulators, but a potential role of the nuclear DNA position remains unclear. We made use of the prominent structure and well-defined heterochromatic landscape of mouse pericentric chromosome domains as a well-studied example of late replicating constitutive heterochromatin. We established a method to manipulate its nuclear position and evaluated the effect on replication timing, DNA compaction and epigenetic composition. Using time-lapse microscopy, we observed that constitutive heterochromatin, known to replicate during late S-phase, was replicated in mid S-phase when repositioned to the nuclear periphery. Out-of-schedule replication resulted in deficient post-replicative maintenance of chromatin modifications, namely silencing marks. We propose that repositioned constitutive heterochromatin was activated in trans according to the domino model of origin firing by nearby (mid S) firing origins. In summary, our data provide, on the one hand, a novel approach to ...Continue Reading

References

May 2, 1997·Science·M K RaghuramanW L Fangman
Apr 18, 2000·The Journal of Cell Biology·H LeonhardtM C Cardoso
Nov 28, 2002·Molecular Cell·Maria VogelauerMichael Grunstein
Jun 9, 2005·The Journal of Cell Biology·Alessandro BreroM Cristina Cardoso
Oct 25, 2006·Nature Methods·Ulrich RothbauerHeinrich Leonhardt
Nov 1, 2006·Journal of Cell Science·Kazuhiro MaeshimaNaoko Imamoto
Aug 21, 2007·Nucleic Acids Research·Beatrice I LindhoutBert J van der Zaal
Oct 24, 2007·Molecular & Cellular Proteomics : MCP·Ulrich RothbauerHeinrich Leonhardt
Feb 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Adrian Salic, Timothy J Mitchison
Aug 2, 2008·Genome Research·Shlomit Farkash-AmarItamar Simon
Mar 10, 2009·Genes & Development·Michaela SchwaigerDirk Schübeler
Dec 17, 2009·Nature Structural & Molecular Biology·Axel KirchhoferUlrich Rothbauer
Mar 4, 2010·Journal of Cell Science·Hani EbrahimiShin-ichiro Hiraga
May 4, 2010·Genome Research·Michaela SchwaigerDirk Schübeler
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Vadim O ChaginM Cristina Cardoso
Mar 3, 2011·Nature Communications·Corella S Casas-DelucchiM Cristina Cardoso
Sep 13, 2011·Nucleic Acids Research·Corella S Casas-DelucchiM Cristina Cardoso
Oct 11, 2011·Nucleus·Corella S Casas-Delucchi, M Cristina Cardoso
Mar 1, 2012·Cell Cycle·Christelle CayrouMarcel Méchali
Mar 19, 2013·Developmental Cell·Wei-Hao ShangTatsuo Fukagawa
Apr 23, 2013·Journal of Molecular Biology·Benjamin D Pope, David M Gilbert
Jul 11, 2013·Cold Spring Harbor Perspectives in Biology·Nicholas Rhind, David M Gilbert
Dec 29, 2013·Nucleic Acids Research·Katharina ThanischHeinrich Leonhardt
Jan 21, 2015·The Journal of Cell Biology·Olivier Hyrien

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Citations

Sep 1, 2018·Epigenomes·Claudia C Preston, Randolph S Faustino
Dec 3, 2020·Nucleic Acids Research·Cathia RauschM Cristina Cardoso
Jul 28, 2020·Trends in Genetics : TIG·Patroula NathanailidouZoi Lygerou
May 20, 2021·Nature Communications·Stefano GnanSara C B Buonomo

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