Peripheral viral challenge triggers hippocampal production of inflammatory proteins

Metabolic Brain Disease
Tiffany J Petrisko, Gregory Konat

Abstract

Peripheral viral infections increase seizure propensity and intensity in susceptible individuals. We have modeled this comorbidity by demonstrating that intraperitoneal (ip) injection of the conventional viral mimetic, polyinosinic-polycytidylic acid (PIC), renders the brain hypersusceptible to seizures induced by kainic acid (KA). At the molecular level, the hippocampus, which is the ictal site of KA-induced seizures, exhibits upregulated expression of messages encoding several inflammatory genes. Here, we profiled temporal expression of these genes at the protein level. Briefly, eight-week old female C57BL/6 mice were ip injected with 12 mg/kg of PIC and inflammatory proteins were quantified in the hippocampus and blood by ELISA. We found a robust but transient increase in blood concentration of IL-6, CXCL10, CCL2, CXCL9, CCL7 and CCL12 six hours after PIC challenge. CXCL1, IL1β, TNFα and CXCL2 featured a moderate increase. However, only four chemokines were increased in the hippocampus. CXCL10 showed the highest increase 6-12 h after PIC challenge, and its level dwindled to the baseline by 48 h. CXCL1, CXCl9 and CXCL2 were also transiently elevated but their maximal values were by an order of magnitude lower than the values ...Continue Reading

References

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Citations

Jan 15, 2019·Metabolic Brain Disease·Tiffany J Petrisko, Gregory W Konat
Aug 19, 2021·Metabolic Brain Disease·Tiffany J Petrisko, Gregory W Konat

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Datasets Mentioned

BETA
AK050012.1

Methods Mentioned

BETA
ELISA

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