Perivitelline space of marsupial oocytes: extracellular matrix of the unfertilized oocyte and formation of a cortical granule envelope following the cortical reaction

Molecular Reproduction and Development
Pramila DandekarP Talbot

Abstract

The purpose of this study was to characterize the structure of the vestments surrounding unfertilized and cortical granule-reacted oocytes from a marsupial, the grey short-tailed opossum Monodelphis domestica and to determine if a cortical granule envelope (CGE) forms in the perivitelline space (PVS) following the cortical reaction. Unfertilized oocytes collected from mature ovarian follicles and oviducal oocytes that had undergone a cortical reaction were fixed for electron microscopy in the presence of ruthenium red which stabilizes extracellular matrices (ECM) and facilitates demonstration of a CGE. Unfertilized oocytes were surrounded by a zona pellucida and had a PVS which contained a thick ECM comprised of granules and filaments. This matrix appeared to attach to the oolemma and was structurally similar to matrices reported previously in the PVS of unfertilized oocytes from eutherian mammals and two other marsupials, the Virginia opossum and the fat-tailed dunnart. The cortex of unfertilized oocytes contained cortical granules which were absent in oocytes recovered from the oviducts of mated females. Oviducal oocytes which lacked cortical granules exhibited a new coat within the PVS between the zona pellucida and the tips...Continue Reading

References

Aug 1, 1992·Journal of Reproduction and Fertility·K E MateJ C Rodger
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Oct 1, 1985·The Journal of Experimental Zoology·E G CothranJ L Vandeberg
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Dec 1, 1994·Molecular Reproduction and Development·T Hoodbhoy, P Talbot

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Citations

Jan 4, 2001·Molecular Reproduction and Development·T Hoodbhoy, P Talbot
Oct 16, 2009·Journal of the Royal Society, Interface·Morteza KhalilianPoopak Eftekhari Yazdi
Nov 18, 2011·Reproductive Biology and Endocrinology : RB&E·Min Liu
Oct 13, 2001·Molecular Reproduction and Development·R J Muñoz-GoteraA Mújica
Mar 8, 2006·Reproduction in Domestic Animals = Zuchthygiene·T H TsiligianniA Van Soom
Mar 28, 2006·Current Topics in Developmental Biology·Julian L Wong, Gary M Wessel
Jun 18, 2003·Microscopy Research and Technique·P Talbot, Pramila Dandekar
Jun 9, 2009·Development·Alina P TartiaJay M Baltz

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