Oct 27, 2004

Perlecan in late stages of osteoarthritis of the human knee joint

Osteoarthritis and Cartilage
F Tesche, Nicolai Miosge

Abstract

Disturbances of the proteoglycan metabolism play an essential role in the pathology of osteoarthritis. The extracellular matrix proteoglycan, perlecan, has lately been identified as a cell biological factor in cartilage development and maintenance. We investigated the tissue distribution of perlecan, the relation between the level of the protein and its mRNA and which type of cell, type 1 chondrocytes or elongated secretory type 2 cells, produces perlecan in late stages of osteoarthritis. In 10 patients suffering from late-stage osteoarthritis tissue samples taken from a macroscopically intact area and the area adjacent to the main cartilage defect were investigated. We performed quantitative immunogold histochemistry and in situ hybridization in vivo and determined the level of perlecan mRNA with the help of real-time RT-PCR in native cartilage tissue and in cultured cells. In vivo, an increased level of perlecan protein was found in the area adjacent to the main defect. A 45% rise in the level of perlecan mRNA secreted by elongated secretory type 2 cells in comparison to type 1 chondrocytes was detected. Type 2 cells also translated the highest levels of perlecan to be deposited mainly in the pericellular matrix, and also in ...Continue Reading

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Citations

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Pathogenic Aspects
Abnormal Degeneration
Pathogenesis
Immunohistochemistry
Phosphoric Monoester Hydrolases
Fissure
Degenerative Polyarthritis
Glycosaminoglycans
Proteoglycan

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