PMID: 1195350Jan 1, 1975Paper

Permeability characteristics of the adipocyte cell membrane and partitioning characteristics of the adipocyte triglyceride core

The Journal of Membrane Biology
B C Sherill, J M Dietschy

Abstract

The unidirectional rates of passive permeation of a homologous series of saturated fatty acids and bile acids into rat epididymal adipocytes were measured to determine the permeability characteristics of this mammalian cell membrane. For fatty acids containing 5 to 12 carbon atoms the logarithm of the permeability coefficient was a linear function of the number of carbons in the fatty acid chain: fatty acids with less than five carbon atoms showed anomalously high permeabilities. Using the data for the fatty acids with 5 to 12 carbon atoms, the incremental free energy of transfer (delta delta F w leads to l) of the -CH2 moiety from the aqueous environment into the fat cell was calculated to equal -547 cal mole-1. The delta delta F w leads to l of the -OH moiety calculated from data using bile acids as the probe molecules was +1,225 cal mole-1. After rupturing the fat cells by freeze-thawing, partition ratios also were measured between bubber and the lipid phase of the adipocyte core using both the fatty acid series and a series of terminal diols as probe molecules. Using these partition ratios delta delta F w leads to l for the -CH2 and -OH substituent groups was calculated to equal -830 and +2,070 cal mole-1, respectively. On ...Continue Reading

References

Jul 6, 1973·Biochimica Et Biophysica Acta·M Kondo, M Kasai
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Dec 1, 1971·The Journal of Membrane Biology·A P Smulders, E M Wright

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Citations

Jan 1, 1984·The Journal of Membrane Biology·A Walter, J Gutknecht
Jan 1, 1983·Comparative Biochemistry and Physiology. A, Comparative Physiology·A B ThomsonW M Weinstein
Nov 24, 2006·Journal of Applied Physiology·Matthew J LayeFrank W Booth
Aug 14, 1980·Biochimica Et Biophysica Acta·S E Schwartz, J M Dietschy
Jun 12, 1987·Biochimica Et Biophysica Acta·H Westergaard
Jul 11, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Frits Kamp, James A Hamilton

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