PMID: 2492515Feb 1, 1989Paper

Permeability of Rickettsia prowazekii to NAD

Journal of Bacteriology
W H Atkinson, H H Winkler

Abstract

Rickettsia prowazekii accumulated radioactivity from [adenine-2,8-3H]NAD but not from [nicotinamide-4-3H]NAD, which demonstrated that NAD was not taken up intact. Extracellular NAD was hydrolyzed by rickettsiae with the products of hydrolysis, nicotinamide mononucleotide and AMP, appearing in the incubation medium in a time- and temperature-dependent manner. The particulate (membrane) fraction contained 90% of this NAD pyrophosphatase activity. Rickettsiae which had accumulated radiolabel after incubation with [adenine-2,8-3H]NAD were extracted, and the intracellular composition was analyzed by chromatography. The cells contained labeled AMP, ADP, ATP, and NAD. The NAD-derived intracellular AMP was transported via a pathway distinct from and in addition to the previously described AMP translocase. Exogenous AMP (1 mM) inhibited uptake of radioactivity from [adenine-2,8-3H]NAD and hydrolysis of extracellular NAD. AMP increased the percentage of intracellular radiolabel present as NAD. Nicotinamide mononucleotide was not taken up by the rickettsiae, did not inhibit hydrolysis of extracellular NAD, and was not a good inhibitor of the uptake of radiolabel from [adenine-2,8-3H]NAD. Neither AMP nor ATP (both of which are transported)...Continue Reading

References

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Citations

Jan 1, 1990·Annals of the New York Academy of Sciences·R R Speed, H H Winkler
Jun 7, 2011·Applied and Environmental Microbiology·Aimee M TuckerDavid O Wood
Jul 15, 1997·European Journal of Biochemistry·B ChowdhuryS K Bose
May 6, 2003·Journal of Bacteriology·Aimee M TuckerDavid O Wood
Jul 17, 1999·Biochimica Et Biophysica Acta·M F Alexeyev, H H Winkler
Mar 1, 1991·Microbiological Reviews·J W Moulder

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