Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy.

BMC Microbiology
Mena CiminoLeiria Salazar

Abstract

The establishment of the cellular localization of proteins in M. tuberculosis will provide of valuable information for the identification of new drug/vaccine/diagnostic targets. Cytolocalization by inmunofluorescence microscopy has been limited in mycobacteria because to difficulties in effectively permeabilize it. A treatment combining lysozyme with triton X-100 was found to be an effective permeabilization method of the mycobacterial envelope. A rapid and simple permeabilization protocol has been successfully assessed in pure cultures of both Mycobacterium smegmatis and Mycobacterium tuberculosis H37Rv. This method can be successful used in the cytolocalization of proteins by immunolabeling.

References


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Citations

Jun 17, 2009·Nucleic Acids Research·Debashree BasuShekhar C Mande
Aug 26, 2010·Academic Medicine : Journal of the Association of American Medical Colleges·John Q YoungPatricia S O'Sullivan
Oct 28, 2011·Academic Medicine : Journal of the Association of American Medical Colleges·Alec B O'ConnorDonald R Bordley
Jul 28, 2013·Academic Medicine : Journal of the Association of American Medical Colleges·Lisa M RoshetskyVineet M Arora
Jun 12, 2014·Nature Communications·Tuhin BhowmickValakunja Nagaraja
Nov 28, 2017·PLoS Genetics·Katherine M MannChristina L Stallings
Aug 3, 2018·Journal of Clinical Microbiology·Ahmed LoukilMichel Drancourt
Mar 26, 2011·Journal of Clinical Laboratory Analysis·Thomas S LawsonJames A Piper
Jan 26, 2021·Journal of Microbiological Methods·Patrick M Richards, Timothy E Mattes

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

Metamorph

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