Permeant anions control gating of calcium-dependent chloride channels

The Journal of Membrane Biology
Patricia Perez-CornejoJorge Arreola

Abstract

The effects of external anions (SCN(-), NO3-, I(-), Br(-), F(-), glutamate, and aspartate) on gating of Ca(2+)-dependent Cl(-) channels from rat parotid acinar cells were studied using the whole-cell configuration of the patch-clamp technique. Shifts in the reversal potential of the current induced by replacement of external Cl(-) with foreign anions, gave the following selectivity sequence based on permeability ratios ( P(x)/ P(Cl)): SCN(-)>I(-)>NO3->Br(-)>Cl(-)>F(-)>aspartate>glutamate. Using a continuum electrostatic model we calculated that this lyotropic sequence resulted from the interaction between anions and a polarizable tunnel with an effective dielectric constant of approximately 23. Our data revealed that anions with P(x)/P(Cl) > 1 accelerated activation kinetics in a voltage-independent manner and slowed deactivation kinetics. Moreover, permeant anions enhanced whole-cell conductance ( g, an index of the apparent open probability) in a voltage-dependent manner, and shifted leftward the membrane potential- g curves. All of these effects were produced by the anions with an effectiveness that followed the selectivity sequence. To explain the effects of permeant anions on activation kinetics and g(Cl) we propose that t...Continue Reading

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Citations

Apr 17, 2008·The Journal of Membrane Biology·Alaka SrivastavaJames E Melvin
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Qinghuan XiaoH Criss Hartzell
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Jan 6, 2016·The Journal of Physiology·Silvia Cruz-RangelJorge Arreola
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Mar 21, 2021·Journal of Molecular Biology·Valeria KalienkovaCristina Paulino

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