PMID: 9533651Apr 9, 1998Paper

Permeation and pathways of human calcitonin (hCT) across excised bovine nasal mucosa

Peptides
S LangHans P Merkle

Abstract

In vitro permeation of human calcitonin (hCT), salmon calcitonin (sCT), and the somatostatin analog octreotide (SMS) through excised bovine nasal mucosa was studied applying donor/receiver experiments and confocal laser scanning microscopy. Permeabilities of gonadorelin, buserelin, Hoe013, and of thymopoietin fragments TP5 and TP4 were also included. Apparent permeability coefficients (Peff) ranged between 4 x 10(-5) (SMS) and 1.7 x 10(-5) cm s(-1) (TP4). Such Peff are typical for leaky-type airway epithelia. The order of permeabilities was: SMS > hCT, sCT > buserelin, Hoe013 > TP5 > TP4, LHRH. The relatively high permeability of hCT and sCT contrasted to their high molecular weight. At 37 degrees C, the permeability of hCT from mucosal to serosal (m-to-s) was found two-fold higher (p < 0.05) than from serosal to mucosal (s-to-m). Controls using 3H-mannitol showed equal permeabilities in both directions. At 4 degrees C, permeation of hCT was reduced but equal in both directions (m-to-s and s-to-m). As evaluated by confocal laser scanning microscopy, uptake studies with FITC-18-hCT revealed intracellular fluorescence in the epithelial cells, at 10 min/10 microM exposure in the form of fluorescent vesicles. By combination of thes...Continue Reading

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Citations

Aug 6, 2011·Pharmaceutical Research·Leonie BaginskiCarsten Ehrhardt
Feb 22, 2012·European Journal of Drug Metabolism and Pharmacokinetics·Hefei ZhangMaureen D Donovan
Dec 29, 2006·Drug Development and Industrial Pharmacy·Alexander GreimelMauro D'Antonio
Jul 6, 2004·The Journal of Pharmacy and Pharmacology·Kavitha N Koushik, Uday B Kompella
Oct 3, 2003·Journal of Molecular Recognition : JMR·Ulrike KraussAnnette G Beck-Sickinger
Apr 29, 2008·The Laryngoscope·Raanan Cohen-KeremIan Witterick
Aug 7, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Rachel Tréhin, Hans P Merkle
Jun 13, 2015·BioMed Research International·Chien-Jung ChenMargaret Dah-Tsyr Chang
Dec 29, 2005·Biochimica Et Biophysica Acta·Robert RennertInes Neundorf
May 8, 2016·International Journal of Pharmaceutics·Anne M Dolberg, Stephan Reichl
Aug 1, 2010·Biomolecular Concepts·Jamie BrugnanoAlyssa Panitch

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