Permeation of Hydrophilic Molecules across Glycated Skin Is Differentially Regulated by the Stratum Corneum and Epidermis-Dermis

Biological & Pharmaceutical Bulletin
Mami Yokota, Yoshihiro Tokudome

Abstract

The effects of glycation on skin permeation and accumulation of compounds were evaluated using an in vitro glycated skin model. Glycation of the skin of hairless mice was induced using vertical diffusion cells and incubation with phosphate-buffered saline containing 50 mM glyoxal for 24 h. Flux and accumulation in the skin were determined by applying hydrophilic and lipophilic molecules (Sodium fluorescein; FL-Na and Nile red, respectively) to this in vitro glycated skin model. Furthermore, to investigate the effect of glycation on epidermal-dermal barrier properties, we conducted diffusion experiments with FL-Na and fluorescein isothiocyanate-dextran using stratum corneum (SC)-stripped glycated skin. The in vitro glycated skin model demonstrated characteristic glycation alterations like a yellowish change in skin color and surface roughness. For low-molecular weight (MW) hydrophilic molecules, flux across glycated full-thickness skin was higher than that across normal skin, although there was no difference with lipophilic molecules. However, glycated epidermis-dermis showed lower flux, and the difference increased with the MW of the compound. Furthermore, the amount of high-MW hydrophilic molecules accumulated in glycated epid...Continue Reading

References

Feb 13, 1986·The New England Journal of Medicine·V M MonnierR R Kohn
Jun 6, 2000·Experimental Dermatology·J D Bos, M M Meinardi
Sep 8, 2000·The Journal of Biological Chemistry·N VerzijlJ M TeKoppele
Oct 6, 2000·Biochimica Et Biophysica Acta·C SadyR H Nagaraj
Oct 14, 2006·The Journal of Investigative Dermatology·Ivone JakasaSanja Kezic
Nov 11, 2008·Nature Biotechnology·Mark R Prausnitz, Robert Langer
Aug 25, 2010·European Journal of Cell Biology·Nina KirschnerJohanna M Brandner
Jun 29, 2011·Biochimica Et Biophysica Acta·Keigo KawabataYoshinori Sugiyama
Jul 30, 2011·Journal of Dermatological Science·Yuki OguraTetsuji Hirao
Aug 23, 2012·Journal of Applied Toxicology : JAT·Audris ChiangHoward I Maibach
Dec 1, 2012·Pharmaceutical Research·Samantha N AndrewsMark R Prausnitz
Mar 8, 2013·Dermato-endocrinology·Paraskevi Gkogkolou, Markus Böhm
Apr 19, 2013·The Journal of Investigative Dermatology·Julia S LeiboldPeter Angel
Jan 8, 2014·Expert Opinion on Drug Delivery·Carl M SchoellhammerRobert Langer

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