Abstract
Studies of the nasal permeation of small molecules (< 1000 Da) have yielded important information about the integrity of the human airway mucosa in health and disease. In this study, we used a much larger tracer molecule, polysucrose (PS) 15,000 (approx. 14,700 Da), to predict the mucosal permeation of inhalational allergens. PS 15,000 (50 mg/ml; 15 ml), with or without a detergent type of permeation enhancer (dioctyl sodium sulfosuccinate 10 mg/ml), was maintained for 15 min in one nasal cavity of 12 healthy nonatopic subjects by employment of a nasal-pool device. Permeation as determined by the 24-h urine recovery of PS (micro-ELISA analysis assay) was expressed as percentage of nasal instillate. Mean baseline permeation was 0.044% (range 0.009-0.250%). In the presence of the detergent, permeation increased to 0.600% (range 0.007-2.260%) (P < 0.01). After oral intake of 750 mg of PS 15,000 (50 micrograms/ml; 15 ml), the 24-h urinary recovery was 0.013% (range 0.004-0.023%). Our study thus demonstrates a measurable baseline permeation of PS 15,000, an elevated permeation rate in the presence of an epithelium-damaging detergent molecule, and a negligible permeation by the oral route. These properties support the utility of PS 1...Continue Reading
References
Jan 1, 1992·International Archives of Allergy and Immunology·H OmanS G Johansson
Oct 1, 1991·Thorax·L GreiffC G Persson
Jan 1, 1991·Acta Oto-laryngologica·L GreiffC G Persson
May 1, 1990·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L GreiffC G Persson
Jan 1, 1985·Journal of Applied Physiology·G F Nieman, C E Bredenberg
Mar 22, 1967·Biochimica Et Biophysica Acta·T C Laurent, K A Granath
Sep 1, 1984·Lancet·I BjarnasonN Veall
Nov 1, 1994·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L GreiffC G Persson
May 1, 1994·The Histochemical Journal·P E TaylorR B Knox
Feb 1, 1994·Thorax·L GreiffC G Persson
Jun 1, 1993·Thorax·L GreiffC G Persson
Sep 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C G PerssonB Gustafsson
Jul 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C G PerssonF Sundler
Jun 1, 1996·Pulmonary Pharmacology·C G PerssonC Svensson
Citations
Nov 24, 1999·Allergy·L Greiff, C G Persson
Oct 9, 2003·Clinical Pharmacokinetics·Stanley S Davis, Lisbeth Illum
May 13, 2017·Immunity, Inflammation and Disease·Caroline SandenJonas S Erjefält
Jun 26, 2001·American Journal of Hematology·M MussoR Scalone
Jul 12, 2005·Oncogene·Christopher J WallickAndré S Bachmann
Mar 30, 2020·Drug Delivery and Translational Research·Prashant G UpadhayaVandana B Patravale