PMID: 9450143Feb 5, 1998Paper

Permeation of polysucrose 15000 across the human nasal mucosa in vivo

Allergy
M AnderssonC G Persson

Abstract

Studies of the nasal permeation of small molecules (< 1000 Da) have yielded important information about the integrity of the human airway mucosa in health and disease. In this study, we used a much larger tracer molecule, polysucrose (PS) 15,000 (approx. 14,700 Da), to predict the mucosal permeation of inhalational allergens. PS 15,000 (50 mg/ml; 15 ml), with or without a detergent type of permeation enhancer (dioctyl sodium sulfosuccinate 10 mg/ml), was maintained for 15 min in one nasal cavity of 12 healthy nonatopic subjects by employment of a nasal-pool device. Permeation as determined by the 24-h urine recovery of PS (micro-ELISA analysis assay) was expressed as percentage of nasal instillate. Mean baseline permeation was 0.044% (range 0.009-0.250%). In the presence of the detergent, permeation increased to 0.600% (range 0.007-2.260%) (P < 0.01). After oral intake of 750 mg of PS 15,000 (50 micrograms/ml; 15 ml), the 24-h urinary recovery was 0.013% (range 0.004-0.023%). Our study thus demonstrates a measurable baseline permeation of PS 15,000, an elevated permeation rate in the presence of an epithelium-damaging detergent molecule, and a negligible permeation by the oral route. These properties support the utility of PS 1...Continue Reading

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Citations

Nov 24, 1999·Allergy·L Greiff, C G Persson
Oct 9, 2003·Clinical Pharmacokinetics·Stanley S Davis, Lisbeth Illum
May 13, 2017·Immunity, Inflammation and Disease·Caroline SandenJonas S Erjefält
Mar 30, 2020·Drug Delivery and Translational Research·Prashant G UpadhayaVandana B Patravale

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