Permissive role of sphingosine on calcium-dependent endocytosis in chromaffin cells.

Pflügers Archiv : European journal of physiology
Juliana M RosaA G García

Abstract

Sphingosine has been shown to modulate neurotransmitter release. Because membrane fusion and fission involve lipid metabolism, we asked here whether sphingosine had a role in regulating endocytosis. To explore this hypothesis, we monitored changes of membrane capacitance (Cm) to study the effects of intracellular sphingosine on membrane retrieval after chromaffin cell stimulation with depolarising pulses (DPs). We found that: (1) sphingosine dialysis through the patch-clamp pipette (SpD) using the whole-cell configuration of the patch-clamp technique (WCC) favours the appearance of a pronounced endocytotic response; (2) SpD-elicited endocytosis was Ca(2+)-dependent but Ba(2+) did not substitute Ca(2+); (3) under WCC, such endocytotic response disappeared with repetitive DPs; (4) in cells preincubated with sphingomyelinase to augment endogenous sphingosine synthesis, and then voltage-clamped under the perforated-patch configuration of the patch-clamp technique (PPC), endocytosis decayed little with repeated stimulation; (5) sphingosine-1-phosphate (S1P), a metabolite of sphingosine, had a meagre effect on endocytosis; and (6) neither dynamin inhibitor dynasore nor calmodulin blocker calmidazolium affected the sphingosine elicite...Continue Reading

References

Feb 1, 1976·Circulation Research·J de ChamplainM R van Ameringen
Mar 1, 1991·Journal of Neuroscience Methods·J RaeM Watsky
Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Lindau, E Neher
Aug 1, 1969·The Journal of Physiology·B Katz, R Miledi
Nov 1, 1968·British Journal of Pharmacology·W W Douglas
Oct 1, 1984·Physiological Reviews·B G Livett
Oct 1, 1980·The Journal of Cell Biology·B Ceccarelli, W P Hurlbut
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Oct 1, 1982·The Journal of Physiology·E M FenwickE Neher
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·C R ArtalejoH C Palfrey
Aug 30, 1996·The Journal of Biological Chemistry·A Blöchl, C Sirrenberg
Jun 1, 1996·Current Opinion in Neurobiology·A W Henkel, W Almers
Dec 31, 1997·The Journal of Cell Biology·C Smith, E Neher
Feb 14, 1998·The Journal of Cell Biology·X ZhaF R Maxfield
Sep 7, 2000·The Journal of Experimental Medicine·A StrelowD Adam
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Cristina R ArtalejoH Clive Palfrey
Aug 7, 2002·British Journal of Pharmacology·Eugen BrailoiuNae J Dun
Mar 14, 2003·Pflügers Archiv : European journal of physiology·Shyue-An ChanCorey Smith
Sep 10, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jeffrey RohrboughKendal Broadie
Oct 4, 2006·Physiological Reviews·Antonio G GarcíaJavier García-Sancho
Feb 28, 2007·Molecular and Cellular Biology·Taketoshi KajimotoShun-ichi Nakamura
Apr 25, 2007·Biochemical and Biophysical Research Communications·Juliana Martins RosaAntonio G García
Jun 6, 2009·Ageing Research Reviews·Fangbai Wu, Pamela J Yao
Jun 12, 2009·American Journal of Physiology. Cell Physiology·Chia-Chang TsaiChien-Yuan Pan

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Citations

Jan 19, 2012·Journal of Molecular Neuroscience : MN·Nathalie C GuérineauEmilio Carbone
Sep 15, 2015·Journal of Neurochemistry·Andrea Crespo-CastrilloAntonio G García
Jan 3, 2012·Cell Calcium·Antonio G GarcíaJavier García-Sancho
Jun 25, 2013·Molecular and Cellular Endocrinology·Ajda FlaškerRobert Zorec
Mar 7, 2021·International Journal of Molecular Sciences·Adonis Z WuSheng-Nan Wu

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