Permselectivity of blood follicle barriers in mouse ovaries of the mifepristone-induced polycystic ovary model revealed by in vivo cryotechnique

Reproduction : the Official Journal of the Society for the Study of Fertility
Hong ZhouShinichi Ohno

Abstract

Despite the potential association of polycystic ovary (PCO) syndrome with hemodynamic changes, follicular microenvironment and the involvement of blood follicle barriers (BFB), a histopathological examination has been hampered by artifacts caused by conventional preparation methods. In this study, mouse ovaries of a mifepristone-induced PCO model were morphologically and immunohistochemically examined by in vivo cryotechnique (IVCT), which prevents those technical artifacts. Ovarian specimens of PCO model mice were prepared by IVCT or the conventional perfusion fixation after s.c. injection of mifepristone. Their histology and immunolocalization of plasma proteins, including albumin (molecular mass, 69 kDa), immunoglobulin G (IgG, 150 kDa), inter-alpha-trypsin inhibitor (ITI, 220 kDa), fibrinogen (340 kDa), and IgM (900 kDa), were examined. In the PCO model, enlarged blood vessels with abundant blood flow were observed in addition to cystic follicles with degenerative membrana granulosa. The immunolocalization of albumin and IgM in the PCO model were similar to those in normal mice. Albumin immunolocalized in the blood vessels, interstitium or follicles, and IgM was mostly restricted within the blood vessels. In contrast, immun...Continue Reading

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Citations

Jul 30, 2010·Journal of Electron Microscopy·Shinichi OhnoYasuhisa Fujii
May 27, 2011·Acta Histochemica Et Cytochemica·Liye ShiShinichi Ohno
Mar 5, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Nobuo TeradaShinichi Ohno
May 8, 2015·Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere·T Stankiewicz
Feb 18, 2012·Biology of Reproduction·Kirsty A WaltersDavid J Handelsman

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