PMID: 109078Dec 1, 1978

Peromyscus alcohol dehydrogenase: lack of cross-reacting material in enzyme-negative animals

Biochemical Genetics
K G Burnett, M R Felder

Abstract

Two forms of alcohol dehydrogenase (ADH), coded by allelic genes, have been purified to homogeneity from Peromyscus. Monospecific antisera to the purified enzymes have been raised in rabbits. These antisera fail to detect cross-reacting material in the liver of ADH-negative animals on Ouchterlony plates. Immuno-titration of anti-ADH antiserum with ADH in liver extracts from AdhS/AdhS and AdhS/AdhN animals results in identical equivalence points, again suggesting the absence of cross-reacting material coded by the AdhN allele. Over a wide range of anti-ADH antiserum dilutions, radiolabeled protein was not immunoprecipitable from liver extracts of AdhN/AdhN animals. These immunochemical tests, in conjunction with previous studies, suggest that the AdhN allele in Peromyscus does not produce inactive polypeptide in normal levels that bears immunological determinants similar to those of the fast and slow ADH isozymes.

References

Jun 1, 1978·Biochemical Genetics·K G Burnett, M R Felder
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Jan 1, 1968·International Archives of Allergy and Applied Immunology·J G Feinberg, C W Hill
Feb 9, 1968·Science·R N FeinsteinB N Jaroslow
Jun 1, 1967·Biochemical Genetics·J G Scandalios

Citations

May 9, 2012·Genetics Research International·Kimberly R ShorterPaul B Vrana
Nov 27, 1985·Biochemical and Biophysical Research Communications·J A Handler, R G Thurman
Nov 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S A BelinskyR G Thurman

Related Concepts

Alcohol Oxidoreductases
Alleles
Metazoa
Cross Reactions
Immunodiffusion Measurement
Alloenzymes
Peromyscus
Mouse, Swiss

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