PMID: 9807736Nov 10, 1998Paper

Peroral administration of specific antibody enhances carcinogen excretion

Journal of Immunotherapy
M V Rasmussen, L K Silbart

Abstract

Ingested carcinogens may exert effects directly on the gastrointestinal epithelium or after absorption and transport to other tissues. To determine the effect of anti-carcinogen antibody ingestion on dietary carcinogen excretion, a mixture of specific IgA or IgG and the model carcinogen 125I-N-2-(4-hydroxyphenyl-acetamido) fluorene (125I-pHP-AAF) was perorally administered to mice. These mice excreted more total and antibody-bound radiotracer in feces compared with controls given a similar mixture containing nonspecific antibody. In addition, urinary radiotracer excretion was reduced by 96% in specific-antibody dosed mice, indicating reduced gastrointestinal absorption of 125I-pHP-AAF. Reduced radiotracer absorption was also reflected by a 56% reduction in radiotracer content in tissues from mice receiving specific antibody. Other mice received peroral IgA before i.p. injection of 125I-PH-AAF. Specific antibody treatment consistently increased intraluminal radiotracer sequestration, as indicated by the level of total and antibody-bound radiotracer partitioning to aqueous fecal extracts. Similarly, when a mixture of 125I-pHP-AAF and IgG were injected directly into the small intestine, more radioactivity appeared in the feces of ...Continue Reading

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