Peroxidase enzymes regulate collagen extracellular matrix biosynthesis

The American Journal of Pathology
Mark O DeNichiloAndreas Evdokiou

Abstract

Myeloperoxidase and eosinophil peroxidase are heme-containing enzymes often physically associated with fibrotic tissue and cancer in various organs, without any direct involvement in promoting fibroblast recruitment and extracellular matrix (ECM) biosynthesis at these sites. We report herein novel findings that show peroxidase enzymes possess a well-conserved profibrogenic capacity to stimulate the migration of fibroblastic cells and promote their ability to secrete collagenous proteins to generate a functional ECM both in vitro and in vivo. Mechanistic studies conducted using cultured fibroblasts show that these cells are capable of rapidly binding and internalizing both myeloperoxidase and eosinophil peroxidase. Peroxidase enzymes stimulate collagen biosynthesis at a post-translational level in a prolyl 4-hydroxylase-dependent manner that does not require ascorbic acid. This response was blocked by the irreversible myeloperoxidase inhibitor 4-amino-benzoic acid hydrazide, indicating peroxidase catalytic activity is essential for collagen biosynthesis. These results suggest that peroxidase enzymes, such as myeloperoxidase and eosinophil peroxidase, may play a fundamental role in regulating the recruitment of fibroblast and the...Continue Reading

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Citations

Dec 9, 2015·Calcified Tissue International·Mark O DeNichiloAndreas Evdokiou
Sep 20, 2015·The International Journal of Biochemistry & Cell Biology·Vasilios PanagopoulosAndreas Evdokiou
Nov 12, 2016·Molecular and Cellular Endocrinology·Vasilios PanagopoulosAndreas Evdokiou
Mar 6, 2017·International Journal of Oncology·Vasilios PanagopoulosAndreas Evdokiou
Jun 20, 2020·Cell Communication and Signaling : CCS·Sarah B GittoDeborah A Altomare
Feb 6, 2020·Frontiers in Oncology·Veronica FioritoEmanuela Tolosano
Sep 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ye WangDusan Losic
Nov 20, 2020·Journal of Periodontal Research·Priscila A GiovaniKamila R Kantovitz
Dec 15, 2020·Seminars in Cell & Developmental Biology·Amita G GhadgeWendy V Ingman

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