Peroxidasin mediates bromination of tyrosine residues in the extracellular matrix.

The Journal of Biological Chemistry
B. B. BathishC. C. Winterbourn

Abstract

Peroxidasin is a heme peroxidase that oxidizes bromide to hypobromous acid (HOBr), a powerful oxidant that promotes the formation of the sulfilimine crosslink in collagen IV in basement membranes. We investigated whether HOBr released by peroxidasin leads to other oxidative modifications of proteins, particularly bromination of tyrosine residues, in peroxidasin-expressing PFHR9 cells. Using stable isotope dilution LC-MS/MS, we detected the formation of 3-bromotyrosine, a specific biomarker of HOBr-mediated protein modification. The level of 3-bromotyrosine in extracellular matrix proteins from normally cultured cells was 1.1 mmol/mol tyrosine and decreased significantly in the presence of the peroxidasin inhibitor, phloroglucinol. A negligible amount of 3-bromotyrosine was detected in peroxidasin-knockout cells. 3-Bromotyrosine formed both during cell growth in culture and in the isolated decellularized extracellular matrix when embedded peroxidasin was supplied with hydrogen peroxide and bromide. The level of 3-bromotyrosine was significantly higher in extracellular matrix than intracellular proteins, although a low amount was detected intracellularly. 3-Bromotyrosine levels increased with higher bromide concentrations and dec...Continue Reading

References

Nov 1, 1981·European Journal of Biochemistry·R TimplK Kühn
May 17, 2000·The Journal of Clinical Investigation·W WuS L Hazen
Jan 13, 2001·Chemico-biological Interactions·P J O'Brien
Apr 24, 2002·The Journal of Biological Chemistry·Munirathinam SundaramoorthyBilly G Hudson
Sep 5, 2002·Free Radical Biology & Medicine·Ruth E AldridgeAnthony J Kettle
Aug 30, 2005·Archives of Biochemistry and Biophysics·Revathy Senthilmohan, Anthony J Kettle
Apr 13, 2006·Chemical Research in Toxicology·Péter NagyMichael T Ashby
Jan 1, 1934·The Biochemical Journal·M Dixon
Jul 29, 2006·American Journal of Rhinology·Martin J CitardiStanley L Hazen
Jan 30, 2009·Journal of Clinical Biochemistry and Nutrition·Yoji KatoToshihiko Osawa
Jul 11, 2009·The American Journal of Pathology·Zalán PéterfiMiklós Geiszt
Sep 5, 2009·Science·Roberto VanacoreBilly G Hudson
Nov 13, 2009·Free Radical Research·Clare L Hawkins
Dec 8, 2009·Science·Richard O Hynes
Jul 22, 2010·Free Radical Biology & Medicine·Eline ThomsonElizabeth Williamson
Mar 12, 2011·The Journal of Pediatrics·Samuel H WedesStanley L Hazen
Jul 30, 2011·Free Radical Biology & Medicine·Guangjie ChengWilliam M Nauseef
Sep 13, 2011·American Journal of Human Genetics·Kamron KhanManir Ali
Jun 19, 2012·Canadian Journal of Physiology and Pharmacology·Ting-Ting LiJun Peng
Jul 31, 2012·Nature Chemical Biology·Gautam BhaveBilly G Hudson
Sep 15, 2012·Chemistry & Biodiversity·Monika SoudiChristian Obinger
Jan 30, 2013·Trends in Cardiovascular Medicine·Qi-Lin MaJun Peng
Sep 12, 2013·Biochemical and Biophysical Research Communications·Lizhen YangRuizheng Shi
Feb 25, 2015·Free Radical Biology & Medicine·Enikő LázárMiklós Geiszt
May 14, 2016·Oncotarget·Aparna JayachandranSonja J McKeown
Oct 5, 2016·The Journal of Biological Chemistry·Selene Colon, Gautam Bhave
Feb 6, 2017·The Journal of Biological Chemistry·Martina Paumann-PageChristian Obinger
Apr 21, 2017·American Journal of Physiology. Renal Physiology·Gautam BhaveNicholas Ferrell
Mar 25, 2018·Redox Biology·Gábor SirokmányMiklós Geiszt
Apr 8, 2018·Archives of Biochemistry and Biophysics·Boushra BathishChristine C Winterbourn
Jun 29, 2018·Gene·Kerry L Hanmer, Demetra Mavri-Damelin
Jul 10, 2018·Cardiovascular Research·Hayfa MedfaiKarim Zouaoui Boudjeltia
Oct 4, 2018·Journal of the American Society of Nephrology : JASN·A Scott McCallBilly G Hudson
Jan 19, 2020·Archives of Biochemistry and Biophysics·Benjamin SevcnikarChristian Obinger
Jun 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Cuiwen HeHaibo Jiang

❮ Previous
Next ❯

Citations

Aug 1, 2021·Expert Review of Molecular Diagnostics·Martin PehrssonMichael Jonathan Davies

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.