Peroxidative modification of a membrane protein. Conformation-dependent chemical modification of adenine nucleotide translocase in Cu2+/tert-butyl hydroperoxide treated mitochondria

Biochemistry
J Girón-Calle, H H Schmid

Abstract

Peroxidative treatment of rat heart mitochondria results in a gradual increase of the apparent molecular weight of the adenine nucleotide translocase (ANT) by up to 1.2 kDa. ANT isolated from mitochondria treated with 1 mM tert-butyl hydroperoxide and 5-40 microM Cu2+ for 1 h at 37 degrees C exhibited a progressive loss of lysine, cysteine, arginine, and valine residues compared to native ANT. N-Ethylmaleimide, dithiothreitol, and the specific inhibitor of ANT, carboxyatractyloside (CAT), inhibited the peroxidation-induced molecular weight shift without inhibiting lipid peroxidation, which is believed to be the primary cause of the observed ANT modification. Bongkrekic acid, which stabilizes ANT in a conformation different from that brought about by CAT, did not inhibit the ANT molecular weight shift. Dithiothreitol, as well as CAT, was found to protect ANT against most of the losses of amino acid residues, indicating that alteration of sulfhydryl residues is required for chemical modification of, not only cysteine, but also lysine, arginine, and valine. We conclude that the peroxidative modification of ANT is conformation-dependent and involves chemical modification of cysteine as a critical step.

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Citations

Feb 12, 2011·Journal of Bioenergetics and Biomembranes·Julia Toman, Gary Fiskum
Nov 7, 2000·Free Radical Biology & Medicine·I DurotP Athias
Nov 26, 2002·Free Radical Biology & Medicine·Rafael RadiLaura Castro
Jan 11, 2003·Free Radical Biology & Medicine·Hirotaka Imai, Yasuhito Nakagawa
May 31, 2008·Biochimica Et Biophysica Acta·Martin Klingenberg
Dec 19, 2007·Journal of Mass Spectrometry : JMS·Laszlo ProkaiMichael J Forster
Sep 21, 2005·Journal of Cellular Biochemistry·Yoshihiro ShidojiKunio Yagi
Aug 12, 2004·Free Radical Biology & Medicine·Martin D BrandNadeene Parker
Jun 23, 2009·Biochimica Et Biophysica Acta·Martin Klingenberg

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