Abstract
To find the catalytic activities of CYP191A1 from Mycobacterium smegmatis, in which functions of most P450s are unknown, by using a set of reductase systems, peroxides, and various substrates including fatty acids and human drugs. CYP191A1 was functionally expressed in Escherichia coli and purified. Its catalytic activities were examined with fatty acids, chromogenic and fluorogenic substrates, and several human P450 substrates, in the presence of six different types of electron transfer systems, such as rat NADPH-P450 reductase, Candida NADPH-P450 reductase, ferredoxin/ferredoxin reductase, putidaredoxin/putidaredoxin reductase, and peroxides (H2O2 and t-butyl hydroperoxide). The reactions catalyzed by CYP191A1 included the hydroxylation and O-dealkylation of several substrates. CYP191A1 preferentially catalyzes the peroxide-dependent oxidation of various substrates over the reductase-dependent reaction. Its peroxygenase activity may be used an effective biocatalytic tool to synthesize the metabolites of drugs.
References
Nov 1, 1990·Chemical Research in Toxicology·R PeterC S Yang
May 29, 1999·Journal of Bacteriology·P PoupinN Truffaut
May 30, 2001·Biochemical Pharmacology·H M Guardiola-DiazA D Vaz
Feb 5, 2003·Biochemical and Biophysical Research Communications·Colin J JacksonSteven L Kelly
Jan 25, 2005·The Journal of Biological Chemistry·Chul-Ho YunF Peter Guengerich
Feb 9, 2008·Current Opinion in Chemical Biology·Mattijs K JulsingAndreas Schmid
Dec 17, 2008·Antimicrobial Agents and Chemotherapy·Andrew G S WarrilowSteven L Kelly
Jul 29, 2009·Archives of Biochemistry and Biophysics·Hugues OuelletPaul R Ortiz de Montellano
Oct 22, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Keon-Hee KimChul-Ho Yun
Jul 26, 2011·Trends in Biotechnology·Vlada B Urlacher, Marco Girhard
Oct 20, 2011·Chemical Society Reviews·Christopher J C WhitehouseLuet-Lok Wong
Mar 16, 2013·Environmental Microbiology·Esther García-FernándezPaul R Ortiz de Montellano
Feb 7, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Osami Shoji, Yoshihito Watanabe
May 23, 2014·Applied Microbiology and Biotechnology·Rita Bernhardt, Vlada B Urlacher
Mar 21, 2015·The Biochemical Journal·James B Y H BehrendorffElizabeth M J Gillam
Nov 3, 2015·Biochemistry·Daniel J FrankPaul R Ortiz de Montellano