Nov 12, 2003

Peroxide toxicity in conditioned lens epithelial cells--evaluation of multi-defense systems

Experimental Eye Research
W MaA Spector


Immortal murine lens epithelial cells which were conditioned to survive peroxide stress were found to have a remarkable increase in catalase activity as well as lesser changes in a number of other antioxidative defense systems [Invest. Ophthalmol. Vis. Sci. 43 (2002) 3251]. Furthermore, the gene expression of hundreds of other genes was altered. In order to determine the relative importance of catalase, other enzyme systems which maintain the reducing environment of the cell and the involvement of Fenton chemistry, an analysis of the effect of inhibiting catalase, disruption of the cells' reducing environment by inhibition of GSSG reductase (GR) and chelation of metal ion was investigated. It was found that inhibition of catalase caused peroxide resistant cells to die within 48-72 hr when exposed to normally tolerated concentrations of peroxide. If 1,10-phenanthroline (OP), an effective metal ion chelator was present, the cells were not affected by catalase inhibition and survived peroxide stress. Peroxide vulnerable unconditioned control cells were similarly protected by the chelator. The results demonstrate that H2O2 itself has minimal toxicity and that it is the products resulting from interaction with metal ion that produce...Continue Reading

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Mentioned in this Paper

Hydrogen Peroxide
Glutathione Disulfide
Squamous Transitional Epithelial Cell Count
Ophthalmology Specialty
Chelator (genus)
1,10-phenanthroline, zinc salt
Oxidative Stress
Chelating Agents

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