Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays.

Journal of Assisted Reproduction and Genetics
Phoebe M HughesCharles C Coddington

Abstract

To determine the relative sensitivities of the 1 and 2-cell mouse embryo assays (MEA) and the human sperm motility assay (HSMA) for peroxides in mineral oil. The effect of peroxide on blastocyst cell number and apoptosis was also studied. One and two-cell MEA and HSMA were performed using mineral oil containing cumene hydroperoxide (CH). The 1-cell MEA was twice as sensitive as the 2-cell MEA and 20-times more sensitive than the HSMA for CH in mineral oil. The sensitivity of the 1-cell MEA doubled when embryos were cultured individually versus group culture. CH decreased blastocyst cell number in a dose dependent manner. Individually cultured 1-cell embryos had the highest sensitivity for peroxides in mineral oil. Current quality control assays, including group cultured murine embryos and human sperm motility, have limited sensitivity for peroxides in mineral oil and may not detect levels of peroxides that cause sub-lethal cellular damage.

References

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Citations

Apr 12, 2013·Human Reproduction Update·David MortimerCharles H Muller
May 11, 2010·Fertility and Sterility·Dean E MorbeckDavid L Walker
Aug 30, 2016·Reproductive Biomedicine Online·Rebecca L Kelley, David K Gardner
Feb 14, 2020·Human Reproduction·L DelarocheP Humaidan
Jan 9, 2017·Journal of Assisted Reproduction and Genetics·Alessandra J AinsworthDean E Morbeck
Apr 14, 2020·Journal of Assisted Reproduction and Genetics·Navid Esfandiari, Ashley Gubista
Feb 14, 2014·Reproductive Medicine and Biology·Manami NishioEimei Sato
Jan 15, 2021·Zygote : the Biology of Gametes and Early Embryos·Sam Zarbakhsh
Feb 26, 2021·Therapeutic Advances in Reproductive Health·Romualdo SciorioSandro C Esteves

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