Peroxiredoxin 2, glutathione peroxidase, and catalase in the cytosol and membrane of erythrocytes under H2O2-induced oxidative stress

Free Radical Research
Susana RochaAlice Santos-Silva

Abstract

Erythrocytes are continuously exposed to risk of oxidative injury due to oxidant oxygen species. To prevent damage, they have antioxidant agents namely, catalase (Cat), glutathione peroxidase (GPx), and peroxiredoxin 2 (Prx2). Our aim was to contribute to a better understanding of the interplay between Prx2, Cat, and GPx under H2O2-induced oxidative stress, by studying their changes in the red blood cell cytosol and membrane, in different conditions. These three enzymes were quantified by immunoblotting. Malondialdehyde, that is, lipoperoxidation (LPO) in the erythrocyte membrane, and membrane-bound hemoglobin (MBH) were evaluated, as markers of oxidative stress. We also studied the erythrocyte membrane protein profile, to estimate how oxidative stress affects the membrane protein structure. We showed that under increasing H2O2 concentrations, inhibition of the three enzymes with or without metHb formation lead to the binding of Prx2 and GPx (but not Cat) to the erythrocyte membrane. Prx2 was detected mainly in its oxidized form and the linkage of metHb to the membrane seems to compete with the binding of Prx2. Catalase played a major role in protecting erythrocytes from high exogenous flux of H2O2, since whenever Cat was activ...Continue Reading

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Citations

Jun 25, 2016·Oxidative Medicine and Cellular Longevity·Jaieun Kim, Young-Sam Keum
Oct 21, 2016·Free Radical Research·Simone B BayerChristine C Winterbourn
Apr 11, 2017·Archives of Biochemistry and Biophysics·Florencia AmenLeonor Thomson
Nov 22, 2019·Journal of Cellular and Molecular Medicine·Hao WuYanjie Lu
Dec 5, 2018·Food Science & Nutrition·Pan ZhaoHaiji Sun
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Aug 19, 2018·Pflügers Archiv : European journal of physiology·Su-Kyung ShinDae-Kyu Song
Aug 7, 2020·American Journal of Physiology. Cell Physiology·Bei ChenWenxue Tang
Jan 1, 2021·International Journal of Molecular Sciences·Sophida PhuthongSilvia M Arribas
Dec 25, 2019·Biochimica Et Biophysica Acta. Biomembranes·Susana RochaAlice Santos-Silva
Aug 18, 2021·Biochimica Et Biophysica Acta. Biomembranes·Rafaela LopesFátima Paiva-Martins
Sep 12, 2020·ACS Chemical Biology·Gaurango ChakrabartyGovindasamy Mugesh

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