Peroxiredoxin 2 is a novel autoantigen for anti-endothelial cell antibodies in systemic vasculitis.

Clinical and Experimental Immunology
R KarasawaT Kato

Abstract

Anti-endothelial cell antibodies (AECA) have been frequently detected in systemic vasculitis, which affects blood vessels of various sizes. To understand the pathogenic roles of AECA in systemic vasculitis, we attempted to identify target antigens for AECA comprehensively by a proteomic approach. Proteins extracted from human umbilical vein endothelial cells (HUVEC) were separated by two-dimensional electrophoresis, and Western blotting was subsequently conducted using sera from patients with systemic vasculitis. As a result, 53 autoantigenic protein spots for AECA were detected, nine of which were identified by mass spectrometry. One of the identified proteins was peroxiredoxin 2 (Prx2), an anti-oxidant enzyme. Frequency of anti-Prx2 autoantibodies, measured by enzyme-linked immunosorbent assay (ELISA), was significantly higher in systemic vasculitis (60%) compared to those in collagen diseases without clinical vasculitis (7%, P < 0·01) and healthy individuals (0%, P < 0·01). Further, the titres changed in parallel with the disease activity during time-courses. The presence of anti-Prx2 autoantibodies correlated significantly with elevation of serum d-dimers and thrombin-antithrombin complex (P < 0·05). Immunocytochemical anal...Continue Reading

References

Nov 1, 1990·Clinical and Experimental Immunology·G FramptonJ S Cameron
Jan 1, 1990·Clinical and Experimental Immunology·G FerraroG Balestrieri
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
Feb 1, 1994·Arthritis and Rheumatism·J C JennetteC G Kallenberg
Sep 1, 1993·Annals of the Rheumatic Diseases·S BashirP G Winyard
May 1, 1993·Clinical Immunology and Immunopathology·A O AydìntugG R Hughes
Jan 1, 1997·The Medical Clinics of North America·M C Sneller, A S Fauci
Nov 20, 1997·The New England Journal of Medicine·J C Jennette, R J Falk
Feb 4, 1998·Current Opinion in Rheumatology·P A Merkel
Mar 25, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·G KrishnaswamyD S Chi
Jan 19, 2000·The Journal of Experimental Medicine·M J NicklinG W Duff
Jun 28, 2002·Cleveland Clinic Journal of Medicine·Jörg J Goronzy, Cornelia M Weyand
Jan 9, 2003·Trends in Biochemical Sciences·Zachary A WoodLeslie B Poole
Dec 21, 2004·Journal of Korean Medical Science·Hyo Seok ChungYoung Hwan Lee
Jan 25, 2005·Microbiology and Immunology·Rie KarasawaTomohiro Kato
Jun 28, 2006·Arthritis and Rheumatism·Sunil Kumar ChauhanSoniya Nityanand
Dec 29, 2007·Journal of Autoimmunity·C G M Kallenberg
Jan 23, 2008·Clinics·Romy Christmann SouzaCélio Roberto Gonçalves
Jun 9, 2009·The Journal of Allergy and Clinical Immunology·Atul Khasnis, Carol A Langford
Jun 11, 2009·Best Practice & Research. Clinical Rheumatology·David Jayne
Jul 10, 2009·Mediators of Inflammation·Sophie OhlssonMårten Segelmark

❮ Previous
Next ❯

Citations

May 23, 2012·Antioxidants & Redox Signaling·Samuel LeeRichard T Lee
Dec 8, 2010·Expert Opinion on Biological Therapy·Rie KarasawaTomohiro Kato
Oct 19, 2011·Microbiology and Immunology·Mikiya FujiedaHiroshi Wakiguchi
Apr 15, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Alexis RégentLuc Mouthon
Feb 22, 2001·Seminars in Arthritis and Rheumatism·V M Martinez-TaboadaV Rodriguez-Valverde
Jul 28, 2015·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Rie Karasawa, James N Jarvis
Mar 11, 2017·Human Vaccines & Immunotherapeutics·Luisa Berenise Gamez-GonzalezMarco Yamazaki-Nakashimada
Aug 17, 2018·Clinical and Experimental Immunology·J CuiH Du
Aug 7, 2019·Current Opinion in Rheumatology·Rie Karasawa, James N Jarvis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.