Peroxiredoxin 6 is upregulated in bovine oocytes and cumulus cells during in vitro maturation: role of intercellular communication

Biology of Reproduction
Gregory LeyensIsabelle Donnay

Abstract

Peroxiredoxins are peroxidases involved in antioxidant defense and intracellular signaling. Expression of transcripts coding for peroxiredoxin 6 (PRDX6) has been previously described to be upregulated in oocytes after in vitro maturation, a period during which general transcription decreases dramatically in oocytes. The aim of the present work was to evaluate PRDX6 regulation in bovine cumulus-oocyte complexes in relation to maturation and intercellular communication. PRDX6 expression was analyzed by reverse transcription-PCR and Western blotting in oocytes and cumulus cells before and after in vitro maturation. PRDX6 was found to be upregulated at the mRNA and protein levels in both cell types after maturation. The effect of paracrine and gap junctional communication on PRDX6 expression was then assessed by culturing cumulus clusters in the presence or absence of denuded oocytes. While PRDX6 upregulation in oocytes required intact cumulus-oocyte junctions, the presence of denuded oocytes was necessary but sufficient for the upregulation to occur in cumulus cells. Finally, the influence of recombinant mouse growth differentiation factor-9 (GDF-9) on PRDX6 expression in cumulus cells was studied. GDF-9 induced cumulus expansion ...Continue Reading

References

Feb 1, 1994·Microscopy Research and Technique·D F Albertini, V Rider
Nov 1, 1993·Trends in Biochemical Sciences·W R Engels
Oct 1, 1995·Molecular Reproduction and Development·J H RalphI Wilmut
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·J D Richter
Jun 24, 1999·Molecular Endocrinology·J A ElvinM M Matzuk
Jul 20, 1999·The Journal of Biological Chemistry·A B FisherS I Feinstein
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·J A ElvinM M Matzuk
Jun 8, 2001·The Journal of Biological Chemistry·S P LeeH Z Chae
Dec 6, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·J J Eppig
Jan 5, 2002·Molecular Reproduction and Development·F NuttinckG Charpigny
Feb 9, 2002·Molecular Reproduction and Development·Sofie TangheAart de Kruif
Mar 9, 2002·Reviews in Endocrine & Metabolic Disorders·Xuemei Wu, Martin M Matzuk
Mar 27, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·Alexandra J HarveyJeremy G Thompson
Aug 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Han-Suk KimAron B Fisher
Oct 23, 2002·Biology of Reproduction·Claude RobertMarc-André Sirard
Jan 9, 2003·Trends in Biochemical Sciences·Zachary A WoodLeslie B Poole
May 29, 2003·Molecular and Cellular Endocrinology·S Mazerbourg, A J W Hsueh
Jul 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Han-Suk KimAron B Fisher
Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gregory LeyensBernard Knoops

❮ Previous
Next ❯

Citations

May 12, 2011·Reproduction, Fertility, and Development·Nathan T BainDean H Betts
May 1, 2012·Reproduction, Fertility, and Development·Mohammad Bozlur RahmanAnn Van Soom
Apr 7, 2010·Antioxidants & Redox Signaling·Christoph UferHartmut Kuhn
Dec 15, 2010·Biology of Reproduction·Jinhwan Lim, Ulrike Luderer
May 6, 2005·Reproductive Biology and Endocrinology : RB&E·Antonin BukovskyMichael R Caudle
Jul 5, 2012·Endocrinology·Kristin E BurnumSudhansu K Dey
Sep 17, 2011·Gene Expression Patterns : GEP·Maxwell E R ShaferSashko Damjanovski
Oct 27, 2007·Biochimica Et Biophysica Acta·Yan MengJia-Hao Sha
Sep 5, 2009·Journal of Cellular Biochemistry·Adriano SpreaficoAnnalisa Santucci
Jun 17, 2008·Developmental Biology·Luis CovarrubiasSusana Castro-Obregón
Dec 20, 2005·Molecular Reproduction and Development·Margot Alves Nunes DodeMarc-André Sirard
Jan 10, 2018·Oxidative Medicine and Cellular Longevity·Bettina P MihalasBrett Nixon
Apr 21, 2010·Reproduction in Domestic Animals = Zuchthygiene·R RomarR Dalbiès-Tran
Jun 6, 2009·Reproductive Biomedicine Online·Catherine M H CombellesAshok Agarwal

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad
Amplify
Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.