Peroxiredoxin IV protects cells from oxidative stress by removing H2O2 produced during disulphide formation.

Journal of Cell Science
Timothy J Tavender, Neil J Bulleid

Abstract

Disulphide formation within the endoplasmic reticulum (ER) requires the activity of the ER oxidase Ero1, and as a consequence, generates hydrogen peroxide. The production of hydrogen peroxide is thought to lead to oxidative stress that ultimately results in apoptosis. Here, we show that mammalian peroxiredoxin IV (PrxIV) metabolises hydrogen peroxide produced by Ero1. We demonstrate that the presence of PrxIV within the ER provides a cytoprotective effect against stresses known to enhance Ero1 activity and perturb ER redox balance. Increased Ero1 activity and production of hydrogen peroxide led to preferential hyperoxidation of PrxIV relative to peroxiredoxins in other cellular compartments. The hyperoxidation was increased by the upregulation of Ero1 and by the expression of a hyperactive Ero1. These findings provide the first evidence for an enzymatic mechanism that facilitates peroxide removal from the ER, and show that the oxidation status of PrxIV acts as a marker for ER oxidative stress.

References

Sep 1, 1999·Free Radical Biology & Medicine·C C Winterbourn, D Metodiewa
Jan 9, 2003·Trends in Biochemical Sciences·Zachary A WoodLeslie B Poole
Apr 26, 2003·Science·Zachary A WoodP Andrew Karplus
Jul 16, 2004·The Journal of Biological Chemistry·Seema Chakravarthi, Neil J Bulleid
Sep 8, 2004·Molecular Cell·Cole M HaynesAntony A Cooper
Oct 21, 2004·Biochemical Society Transactions·L Ellgaard
Oct 28, 2004·The Journal of Biological Chemistry·Catherine E Jessop, Neil J Bulleid
Jun 15, 2005·Annual Review of Biochemistry·Martin Schröder, Randal J Kaufman
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Einav GrossDeborah Fass
Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser
Mar 3, 2007·The Journal of Biological Chemistry·Alexander V PeskinChristine C Winterbourn
Dec 19, 2007·The Journal of Cell Biology·Irina RaykhelLloyd Ruddock
Oct 4, 2008·The EMBO Journal·Christian Appenzeller-HerzogLars Ellgaard
Nov 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jyoti D MalhotraRandal J Kaufman
Dec 25, 2008·The Biochemical Journal·Yoshihito IuchiJunichi Fujii

❮ Previous
Next ❯

Citations

Oct 24, 2012·Protoplasma·Isabel Aller, Andreas J Meyer
Oct 3, 2012·The Journal of Biological Chemistry·Henning Gram HansenLars Ellgaard
Aug 28, 2013·The Journal of Biological Chemistry·Taichi KakihanaKazuhiro Nagata
Aug 23, 2011·Antioxidants & Redox Signaling·Tiziana AnelliRoberto Sitia
Dec 8, 2011·Antioxidants & Redox Signaling·Kazutaka Araki, Kenji Inaba
Dec 31, 2011·Antioxidants & Redox Signaling·Lloyd W Ruddock
Dec 19, 2012·Antioxidants & Redox Signaling·Alberto Bindoli, Maria Pia Rigobello
Apr 22, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·King-Tung ChinDavid Ron
Aug 31, 2011·Cold Spring Harbor Perspectives in Biology·Kazutaka Araki, Kazuhiro Nagata
Jun 23, 2011·Journal of Cell Science·Marcel van LithNeil J Bulleid
Nov 3, 2011·PloS One·Jakob HansenPeter Bross
Dec 25, 2012·International Journal of Molecular Sciences·Bidur BhandaryHan-Jung Chae
Nov 28, 2013·International Journal of Cell Biology·Thomas Ramming, Christian Appenzeller-Herzog
Mar 29, 2014·International Journal of Cell Biology·Jinah ParkSang Won Kang
Apr 19, 2011·Annual Review of Biochemistry·Ineke Braakman, Neil J Bulleid
Jun 10, 2014·FEBS Letters·I Robinson, A B Reddy
Jun 3, 2014·International Journal of Molecular Sciences·Victoriano Pérez-VázquezSergio Encarnación-Guevara
Nov 12, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shu-Xun LiuBo-Lin Zhang
Sep 10, 2013·Seminars in Radiation Oncology·Marianne Koritzinsky, Bradly G Wouters
Feb 5, 2013·Journal of Molecular Biology·Andreas FunknerDavid M Ferrari
Dec 24, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Irina MilisavSamo Ribarič
Nov 27, 2015·Antioxidants & Redox Signaling·Dmitry Bilan, Vsevolod Belousov
Jul 23, 2011·Trends in Biochemical Sciences·Neil J Bulleid, Lars Ellgaard
Nov 27, 2015·Redox Biology·Thomas RammingChristian Appenzeller-Herzog
Dec 23, 2015·Biochimica Et Biophysica Acta·Vladimir I KlichkoSvetlana N Radyuk
Nov 19, 2014·Biochemistry·Arden PerkinsP Andrew Karplus
Sep 19, 2015·Redox Biology·Marta PajaresAntonio Cuadrado
Nov 30, 2012·American Journal of Reproductive Immunology : AJRI·Behrouz Gharesi-FardEskandar Kamali-Sarvestani
Jan 24, 2014·Biochemical Society Transactions·Rachel E MartinNeil J Bulleid
Sep 16, 2011·The Biochemical Journal·Xi WangChih-chen Wang
Mar 20, 2013·Biochimica Et Biophysica Acta·Tali GidalevitzYair Argon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis