PMID: 11309139Apr 20, 2001Paper

Peroxisomal alanine : glyoxylate aminotransferase (AGT1) is a photorespiratory enzyme with multiple substrates in Arabidopsis thaliana

The Plant Journal : for Cell and Molecular Biology
A H Liepman, L J Olsen

Abstract

At least two glyoxylate aminotransferases are hypothesized to participate in the steps of photorespiration located in peroxisomes. Until recently, however, genes encoding these enzymes had not been identified. We describe the isolation and characterization of an alanine : glyoxylate aminotransferase (AGT1, formerly AGT) cDNA from Arabidopsis thaliana. Southern blot analysis confirmed that Arabidopsis AGT1 is encoded by a single gene. Homologs of this class IV aminotransferase are also known in other plants, animals, and methylotrophic bacteria, suggesting an ancient evolutionary origin of this enzyme. AGT1 transcripts were present in all tissues of Arabidopsis, but were most abundant in green, leafy tissues. Purified, recombinant Arabidopsis AGT1 expressed in Escherichia coli catalyzed three transamination reactions using the following amino donor : acceptor combinations: alanine : glyoxylate, serine : glyoxylate, and serine : pyruvate. AGT1 had the highest specific activity with the serine : glyoxylate transamination, and apparent Km measurements indicate that this is the preferred in vivo reaction. In vitro import experiments and subcellular fractionations localized AGT1 to peroxisomes. Sequence analysis of the photorespirato...Continue Reading

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Citations

Mar 3, 2010·Journal of Experimental Botany·Le YuXinxiang Peng
Dec 17, 2009·Plant & Cell Physiology·Makoto Hayashi, Mikio Nishimura
Dec 13, 2005·Plant Physiology·Lars M VollAndreas P M Weber
Sep 5, 2006·Plant Physiology·Daisuke IgarashiChieko Ohsumi
Jul 4, 2012·Acta Crystallographica. Section D, Biological Crystallography·Christopher SayerJennifer Littlechild
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·T Yang, B W Poovaiah
Jun 25, 2014·Antioxidants & Redox Signaling·Peter Geigenberger, Alisdair R Fernie
Jul 7, 2009·Annual Review of Plant Biology·Christine H FoyerGraham Noctor
May 15, 2003·Molecular Membrane Biology·John A ParkesAlison Baker
Nov 26, 2010·FEBS Letters·Ryoung ShinDaniel P Schachtman
Nov 19, 2015·The Plant Journal : for Cell and Molecular Biology·Huili YuanNatal A W van Riel
Dec 3, 2015·Plant & Cell Physiology·Laure GaufichonAkira Suzuki
Mar 13, 2003·The Plant Journal : for Cell and Molecular Biology·Daisuke IgarashiChieko Ohsumi
Apr 12, 2013·Plant Biology·A FlorianA R Fernie
Mar 20, 2013·Plant Biology·M HodgesG Tcherkez
Feb 21, 2013·Plant, Cell & Environment·Qiying ZhouGuangcun He

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