Peroxisomal proteins in rat gametes

Cell Biochemistry and Biophysics
C FigueroaM J Santos

Abstract

Peroxisomes are essential subcellular organelles, which appear to be derived from pre-existing organelles. This biogenetic mechanism assumes the presence of peroxisomes in either or both mammalian gametes (sperms and/or oocytes). In order to test the presence and subcellular localization of peroxisomal proteins in rat sperms and oocytes, the authors carried out fractionation and immunofluorescence experiments. The results showed that rat oocytes contain peroxisome-like structures, which were detected by indirect immunofluorescence, using an antisera against total peroxisomal proteins. In contrast, such structures were not detected in rat sperms, which appear to contain catalase localized in the cell cytosol. The results reported herein show evidence for the first time of the presence of peroxisome-like structures in mammalian oocytes, and provide evidence for the peroxisome biogenesis hypothesis, by division of pre-existing organelles.

Citations

Dec 1, 2005·Histochemistry and Cell Biology·Georg H LüersJürgen Seitz
Nov 1, 2001·Annual Review of Cell and Developmental Biology·P E Purdue, P B Lazarow
Jun 18, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Sarah JansenPeter L Kaye
May 21, 2009·Molecular Reproduction and Development·Stephen M DownsJonathan Klinger
Aug 9, 2006·Molecular Reproduction and Development·Ricardo D Moreno, Carlos P Alvarado
Dec 28, 2007·Cytogenetic and Genome Research·P GrzmilP Burfeind

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