Peroxisome proliferator-activated receptor-γ-mediated polarization of macrophages in Neospora caninum infection

Experimental Parasitology
Xuexiu HeXichen Zhang

Abstract

Neospora caninum is an apicomplexan parasite closely related Toxoplasma gondii, which causes neurological disease and abortion in multiple animal species. Macrophage polarization plays an important role in host immune responses to parasites infection, such as Toxoplasma gondii, Leishmania, Trypanosoma cruzi. However, the dynamics of macrophage polarization, as well as the possible mechanism that regulate macrophage polarization, during N. caninum infection remains unclear. The M1 and M2-phenotypic markers of peritoneal macrophages from mice infected with tachyzoites of Nc-1 were analyzed by flow cytometry (FCM) analysis. Then J774A.1 cells were respectively treated with GW9662 and RGZ, and stimulated by tachyzoites of Nc-1. M1 and M2-phenotypic markers were determined by FCM and ELISA. And the activations of PPAR-γ and NF-κB were determined by Western blotting. In this study, our data showed that macrophages were preferentially differentiated into the M1 type during the acute stage of N. caninum infection, while the level of M2 macrophages significantly increased during the chronic stage of infection. In vitro study, compared with the GW9662 group and RGZ group, N. caninum can promote M2-polarized phenotype through up-regulate ...Continue Reading

Citations

Mar 22, 2019·PLoS Pathogens·Chrissy M Leopold WagerLarry S Schlesinger
May 23, 2019·Frontiers in Immunology·Marta García-SánchezEsther Collantes-Fernández
Aug 21, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Juan CaiHui Liu

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