Peroxisome proliferator-activated receptor gamma-mediated regulation of neural stem cell proliferation and differentiation

The Journal of Biological Chemistry
Koichiro WadaRichard S Blumberg

Abstract

Peroxisome proliferator-activated receptor gamma (PPARgamma) plays an important role in insulin sensitivity, tissue homeostasis, and regulating cellular functions. We found high-level expression of PPARgamma in embryo mouse brain and neural stem cells (NSCs), in contrast to extremely low levels in adult mouse brain. Here, we show that PPARgamma mediates the proliferation and differentiation of murine NSCs via up-regulation of the epidermal growth factor receptor and activation of the ERK pathway. Cell growth rates of NSCs prepared from heterozygous PPARgamma-deficient mouse brains, PPARgamma-RNA-silenced NSCs, and PPARgamma dominant-negative NSCs were significantly decreased compared with those of wild-type NSCs. Physiological concentrations of PPARgamma agonists, rosiglitazone and pioglitazone, stimulated NSC growth, whereas antagonists caused cell death in a concentration-dependent manner via activation of the caspase cascade. The stimulation of cell growth by PPARgamma was associated with a rapid activation of the ERK pathway by phosphorylation and up-regulation of epidermal growth factor receptor and cyclin B protein levels. In contrast, activation of PPARgamma by agonists inhibited the differentiation of NSCs into neurons....Continue Reading

References

May 16, 1997·The Journal of Biological Chemistry·C L Rokos, B J Ledwith
Dec 22, 1999·Current Opinion in Biotechnology·L Michalik, W Wahli
Jun 6, 2000·Nature·S KerstenW Wahli
Aug 23, 2000·The Journal of Clinical Investigation·T Kadowaki
May 23, 2001·Nature Neuroscience·L ContiE Cattaneo
Nov 2, 2001·Nature·S Temple
Dec 4, 2001·Biology of the Cell·C Peyssonnaux, A Eychène
Feb 2, 2002·Experimental Hematology·Satoko Tahara-HanaokaHiromitsu Nakauchi
Apr 27, 2002·Nature Neuroscience·T A Reh
Jun 18, 2002·Journal of Neurochemistry·Thomas ZanderMichael T Heneka
Oct 5, 2002·Cellular and Molecular Life Sciences : CMLS·C H YamR Y C Poon
Oct 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Maria Luisa MartelliAlfredo Fusco
Mar 22, 2003·Experimental Cell Research·Robert N JorissenAntony W Burgess
Jan 22, 2004·Biochemical and Biophysical Research Communications·Asish K SahaNeil B Ruderman
Feb 9, 2006·American Journal of Physiology. Endocrinology and Metabolism·Nathan K LeBrasseurEva Tomas

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Citations

Apr 26, 2008·Journal of Molecular Neuroscience : MN·Martin J LanHusseini K Manji
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chengwei HeJing X Kang
Mar 20, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jorge E ToblliNestor F Gonzalez-Cadavid
Jun 6, 2013·Clinical and Experimental Pharmacology & Physiology·Colin SumnersUlrike M Steckelings
Aug 22, 2007·PPAR Research·Louis CasteillaMamen Carmona
Jul 23, 2008·PPAR Research·Markus P Kummer, Michael T Heneka
Oct 1, 2009·PPAR Research·Sriram RamananMike E Robbins
Aug 14, 2013·Lipids in Health and Disease·Kae HirataYoshinori Kamisaki
Jun 7, 2013·PloS One·Rodrigo A QuintanillaNibaldo C Inestrosa
Nov 21, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sarah C SchockHsiao-Huei Chen
Aug 22, 2009·Experimental & Molecular Medicine·Yangsik Jeong, David J Mangelsdorf
Sep 24, 2014·PPAR Research·Rodrigo A QuintanillaFabián A Cabezas-Opazo
Nov 22, 2011·Differentiation; Research in Biological Diversity·Ali GhoochaniHossein Baharvand
Jul 8, 2008·PPAR Research·Paul D DrewMichael K Racke
Feb 20, 2009·Journal of Neurochemistry·Francesca DoonanThomas G Cotter
Jun 17, 2010·Physiology·Nicolas Rotman, Walter Wahli
Aug 19, 2011·Journal of Neurochemistry·Yanlin WangXin-Min Li

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