Peroxisome proliferator-activated receptor-gamma agonists inhibit the replication of respiratory syncytial virus (RSV) in human lung epithelial cells

Virology
Ralf Arnold, Wolfgang König

Abstract

We have previously shown that peroxisome proliferator-activated receptor-gamma (PPARgamma) agonists inhibited the inflammatory response of RSV-infected human lung epithelial cells. In this study, we supply evidence that specific PPARgamma agonists (15d-PGJ2, ciglitazone, troglitazone, Fmoc-Leu) efficiently blocked the RSV-induced cytotoxicity and development of syncytia in tissue culture (A549, HEp-2). All PPARgamma agonists under study markedly inhibited the cell surface expression of the viral G and F protein on RSV-infected A549 cells. This was paralleled by a reduced cellular amount of N protein-encoding mRNA determined by real-time RT-PCR. Concomitantly, a reduced release of infectious progeny virus into the cell supernatants of human lung epithelial cells (A549, normal human bronchial epithelial cells (NHBE)) was observed. Similar results were obtained regardless whether PPARgamma agonists were added prior to RSV infection or thereafter, suggesting that the agonists inhibited viral gene expression and not the primary adhesion or fusion process.

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Citations

Aug 18, 2010·Viral Immunology·Josep Bassaganya-RieraRaquel Hontecillas
May 1, 2010·Biochemical and Biophysical Research Communications·Yuta WakuiTooru Shimosegawa
Feb 27, 2014·BMB Reports·Tae Hoon Kim, Heung Kyu Lee
Dec 21, 2011·Antiviral Chemistry & Chemotherapy·Pilar NajarroKenneth Powell
Mar 20, 2018·Frontiers in Immunology·Stephanie AscoughChristopher Chiu
Aug 18, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Merilin Al Sharif
May 11, 2018·Science Translational Medicine·Rhiannon B WerderSimon Phipps

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