PMID: 9163348Apr 15, 1997Paper

Peroxisome proliferator-induced acyl-CoA thioesterase from rat liver cytosol: molecular cloning and functional expression in Chinese hamster ovary cells

The Biochemical Journal
S T EngbergL T Svensson

Abstract

We have isolated and cloned a cDNA that codes for one of the peroxisome proliferator-induced acyl-CoA thioesterases of rat liver. The deduced amino acid sequence corresponds to the major induced isoform in cytosol. Analysis and comparison of the deduced amino acid sequence with the established consensus sequences suggested that this enzyme represents a novel kind of esterase with an incomplete lipase serine active site motif. Analyses of mRNA and its expression indicated that the enzyme is significantly expressed in liver only after peroxisome proliferator treatment, but isoenzymes are constitutively expressed at high levels in testis and brain. The reported cDNA sequence is highly homologous to the recently cloned brain acyl-CoA thioesterase [Broustas, Larkins, Uhler and Hajra (1996) J. Biol. Chem. 271, 10470-10476], but subtle differences throughout the sequence, and distinct differences close to the resulting C-termini, suggest that they are different enzymes, regulated in different manners. A full-length cDNA clone was expressed in Chinese hamster ovary cells and the expressed enzyme was characterized. The palmitoyl-CoA hydrolysing activity (Vmax) was induced approx. 9-fold to 1 micromol/min per mg of cell protein, which wa...Continue Reading

Citations

Jun 15, 2004·Experimental Cell Research·Mitsuru IshizukaTakashi Saito
Sep 24, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Malin BoströmJoseph W DePierre
Apr 27, 2001·European Journal of Biochemistry·N SuematsuF Isohashi
Jun 26, 1999·The Journal of Biological Chemistry·V PouponA Benmerah
Jul 1, 2004·Molecular and Cellular Biology·Ruiyin ChuMatthew Davison
Feb 9, 2002·Brain Research. Molecular Brain Research·Yu KuramochiJunji Yamada
Jan 5, 2002·Progress in Lipid Research·Mary C Hunt, Stefan E H Alexson
Jul 9, 1999·Biochimica Et Biophysica Acta·M A DiczfalusyS E Alexson
Jan 23, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Asim K DuttaroyMargaret J Gordon
Feb 4, 2006·Archives of Biochemistry and Biophysics·Mitsuhiro TakagiJunji Yamada

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