Peroxisome Proliferator-induced Transformation of Syrian Hamster Embryo Cells: Influence of Experimental Procedures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
V CrucianiP Vasseur

Abstract

We compared the ability of four hepatic peroxisome proliferators (HPPs) to induce morphological transformation (MT) in Syrian hamster embryo (SHE) cells under different experimental conditions including the composition of the test medium (DMEM at pH7.35 and 7.0, and in LeBoeuf's modified DMEM at pH6.7) and the modalities of exposure. The HPPs studied were two structurally-related hypolipidaemic agents, clofibrate and methyl clofenapate (MCP), an industrial plasticizer, di(2-ethylhexyl)phthalate (DEHP) and one of its primary active metabolite in vivo, mono(2-ethylhexyl)phthalate (MEHP). SHE cells were exposed to the HPP tested either alone, or in sequential treatments with other carcinogens such as benzo[a]pyrene (BaP) or 12-O-tetradecanoyl-phorbol-13-acetate (TPA) in order to study possible interactions. A two-stage exposure assay was applied with DMEM at pH7.35 and 7.0. Structural analogues did not give similar results using the same experimental conditions. Indeed, while MCP was more potent at acidic pH, the transforming potency of clofibrate was higher at pH7.0 and 7.35. DEHP and MEHP also behaved differently: in contrast to DEHP, MEHP was more active at pH6.7 than at pH7.0. The MT induction, resulting from the interaction b...Continue Reading

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J C Barrett, P O Ts'o
Nov 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R J PriceB G Lake
Nov 15, 1990·International Journal of Cancer. Journal International Du Cancer·S O MikalsenT Sanner
Jun 1, 1988·Regulatory Toxicology and Pharmacology : RTP·W T Stott
May 1, 1987·Carcinogenesis·M S Rao, J K Reddy
Nov 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P BentleyH J Wiegand
Dec 21, 1963·Nature·Y BERWALD, L SACHS
Apr 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E Rivedal, U Haddeland
Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C A JonesR W Tennant

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