Peroxisomes contain delta 3,5,delta 2,4-dienoyl-CoA isomerase and thus possess all enzymes required for the beta-oxidation of unsaturated fatty acids by a novel reductase-dependent pathway

Biochemical and Biophysical Research Communications
X Y HeH Schulz

Abstract

The presence of delta 3,5,delta 2,4-dienoyl-CoA isomerase in peroxisomes was demonstrated by determining the subcellular distribution of this enzyme in rat liver. The peroxisomal and mitochondrial forms of the isomerase exhibit similar chain length specificities and they are homologous as indicated by the recognition of the peroxisomal 66-kDa enzyme by an antiserum raised against the mitochondrial 32-kDa isomerase. This report demonstrates that peroxisomes contain all enzymes required for the beta oxidation of unsaturated fatty acids with odd-numbered double bonds by a novel pathway in which double bonds are reductively removed by the NADPH-dependent 2,4-dienoyl-CoA reductase.

Citations

Jan 10, 2002·The Journal of Biological Chemistry·Dongyan ZhangHorst Schulz
Jul 4, 2003·The Journal of Biological Chemistry·Paul A HubbardJung-Ja P Kim
Jul 5, 1996·The Journal of Biological Chemistry·D L LuthriaH Sprecher
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Aug 28, 1999·The Journal of Biological Chemistry·B V GeisbrechtS J Gould
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Related Concepts

Ech1 protein, mouse
2,4-dienoyl-CoA reductase
Metazoa
Cell Fractionation
Centrifugation, Density Gradient
SDS-PAGE
Fatty Acids, Unsaturated
Isomerase
Liver
Glycosomes

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