Peroxodisulfate as a chemical initiator for methacrylate-ester monolithic columns for capillary electrochromatography

Electrophoresis
Amparo Cantó-MirapeixErnesto F Simó-Alfonso

Abstract

Organic monolithic stationary phases for CEC were synthesized in situ in fused-silica capillaries. Polymerization mixtures were composed of butyl methacrylate, ethylene dimethacrylate, and [2-(methacryloyloxy)ethyl]trimethyl ammonium chloride in the presence of a porogenic solvent, using ammonium peroxodisulfate as chemical initiator, and N,N,N',N'-tetramethylethylenediamine to activate the reaction. The influence of the amount of initiator, temperature, and composition of porogenic solvent on the physical and chromatographic properties of monolithic stationary phases has been investigated. A minimum plate height of 14.5 microm was obtained at 18 wt% of 1,4-butanediol in the polymerization mixture. The produced monolithic stationary phases exhibited a good repeatability and batch-to-batch and mixture-to-mixture reproducibility, with RSD values below 5.6% in the electrochromatographic parameters studied. A comparison with columns prepared by thermal initiation with alpha,alpha'-azobisisobutyronitrile (AIBN) was also performed. The most efficient column initiated with peroxodisulfate showed better efficiencies and selectivities than that prepared with AIBN at the same composition mixture.

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Citations

Jul 31, 2014·Analytical and Bioanalytical Chemistry·María Navarro-Pascual-AhuirJosé Manuel Herrero-Martínez
Sep 19, 2009·Journal of Chromatography. a·V Bernabé-ZafónJ M Herrero-Martínez
Sep 1, 2009·Journal of Chromatography. a·Amparo Cantó-MirapeixErnesto F Simó-Alfonso
Oct 10, 2009·Electrophoresis·Virginia Bernabé-ZafónJosé Manuel Herrero-Martínez
Jan 27, 2009·Electrophoresis·Amparo Cantó-MirapeixErnesto F Simó-Alfonso
Jan 27, 2009·Electrophoresis·Amparo Cantó-MirapeixErnesto F Simó-Alfonso
Mar 24, 2010·Electrophoresis·Juan José Baeza-BaezaErnesto F Simó-Alfonso
Oct 24, 2008·Electrophoresis·Amparo Cantó-MirapeixErnesto F Simó-Alfonso
Jun 12, 2009·Electrophoresis·Virginia Bernabé-ZafónJosé Manuel Herrero-Martínez
Sep 10, 2008·Electrophoresis·Amparo Cantó-MirapeixErnesto F Simó-Alfonso
Jun 12, 2009·Electrophoresis·Frantisek Svec

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