PMID: 9191596Jun 1, 1997Paper

Peroxynitrite and oxidative damage in experimental autoimmune uveitis

Investigative Ophthalmology & Visual Science
G S WuN A Rao

Abstract

Results of a previous study demonstrated the presence of superoxide and nitric oxide in experimental autoimmune uveitis (EAU). These two chemical entities have recently been shown to combine rapidly to form one of the most potent known biologic oxidants, peroxynitrite. As an extension of the previous study, the current study was proposed to determine whether peroxynitrite is generated in EAU and the site and extent of any oxidative damage thereby inflicted. Experimental uveitis was induced in Lewis rats with retinal S-antigen. The localization of peroxynitrite was carried out by immunohistochemical detection of its nitration product, nitrotyrosine, using polyclonal rabbit antinitrotyrosine antibody. The lipid peroxides in the membrane were detected by fluorescent labeling of their secondary carbonyl products with 3-hydroxy-2-naphthoic acid hydrazide. This fluorescent product was also visualized by coupling with Fast Blue B. A confocal laser scanning microscope was used for detection. In EAU, the peroxynitrite plaques were observed to concentrate in the photoreceptors but were also visible in some inner retinal areas, including ganglion cell layers, nerve fiber layers, and retinal blood vessels. The lipid peroxides were localize...Continue Reading

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