Peroxynitrite inhibits inducible (type 2) nitric oxide synthase in murine lung epithelial cells in vitro

Free Radical Biology & Medicine
V K RobinsonJ C Hoyt

Abstract

Peroxynitrite, formed by nitric oxide (NO) and superoxide, can alter protein function by nitrating amino acids such as tyrosine, cysteine, trytophan, or methionine. Inducible nitric oxide synthase (Type 2 NOS or iNOS) converts arginine to citrulline, releasing NO. We hypothesized that peroxynitrite could function as a negative feedback modulator of NO production by nitration of iNOS. Confluent cultures of the murine lung epithelial cell line, LA-4 were stimulated with cytokines to express iNOS, peroxynitrite was added, and the flasks sealed. After 3 h, NO in the headspace above the culture was sampled. Peroxynitrite caused a concentration-dependent decrease in NO. Similar results were obtained when 3-morpholinosydnonimine (SIN-1), a peroxynitrite generator, was added to the flasks. PAPA-NONOate, the NO generator, did not affect the headspace NO. Nitration of the iNOS was confirmed by detection of 3-nitrotyrosine by Western blotting. These data suggest a mechanism for inhibition of NO synthesis at inflammatory sites where iNOS, NO, and superoxide would be expected.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 30, 1992·Biochemical and Biophysical Research Communications·N E Rogers, L J Ignarro
Nov 1, 1992·Archives of Biochemistry and Biophysics·H IschiropoulosJ S Beckman
May 1, 1992·Chemical Research in Toxicology·J J Moreno, W A Pryor
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·L N HeissW E Goldman
Feb 15, 1994·Biochemical and Biophysical Research Communications·R A RobbinsJ Polak
Oct 1, 1995·Archives of Biochemistry and Biophysics·M G SalgoW A Pryor
Nov 1, 1993·Circulation Research·G M BugaL J Ignarro
May 15, 1995·FEBS Letters·H Ischiropoulos, A B al-Mehdi
Jun 1, 1995·Archives of Biochemistry and Biophysics·A van der VlietC E Cross
Jan 1, 1995·Free Radical Biology & Medicine·W Vogt
Feb 15, 1993·The Biochemical Journal·R RadiB A Freeman
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·S K KongP B Chock
Jul 1, 1996·Chemical Research in Toxicology·J S Beckman
Mar 1, 1996·Chemical Research in Toxicology·B AlvarezR Radi
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·L A MacMillan-CrowJ A Thompson
Jan 31, 1997·The Journal of Biological Chemistry·A J GowH Ischiropoulos
Apr 15, 1999·Japanese Journal of Pharmacology·T Nakaki, T Fujii
Dec 30, 1999·American Journal of Respiratory Cell and Molecular Biology·E SatoR A Robbins
Mar 29, 2000·The Journal of Biological Chemistry·C I LeeJ L Zweier

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Citations

Jul 13, 2004·Histochemistry and Cell Biology·Leslie Myatt, Xiaolan Cui
Jun 11, 2002·Archives of Biochemistry and Biophysics·E GiannopoulouE Papadimitriou
Sep 18, 2002·Archives of Biochemistry and Biophysics·Brian M MorrisseyDavid M Rodman
Mar 22, 2002·Developmental and Comparative Immunology·James L StaffordMiodrag Belosevic
Jul 20, 2004·Clinical Microbiology Reviews·Ian A ClarkWilliam B Cowden
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Mar 11, 2003·American Journal of Respiratory Cell and Molecular Biology·Hiroki NumanamiRichard A Robbins
Sep 5, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Ekambaram PadminiVenkatraman Uthra
Mar 1, 2010·Environmental Toxicology and Pharmacology·Shanmugam Lakshmi Devi, Carani V Anuradha
Nov 26, 2008·The Science of the Total Environment·E PadminiM Usha Rani
Jun 14, 2005·Redox Report : Communications in Free Radical Research·Sarika SinghM Dikshit
Sep 1, 2012·Toxicology Mechanisms and Methods·Padmini EkambaramLavanya Dhakshinamoorthy
Jun 28, 2011·American Journal of Obstetrics and Gynecology·Laura MazzantiAndrea L Tranquilli
Sep 7, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Natalia A OsnaTerrence M Donohue
Jan 15, 2002·American Journal of Physiology. Cell Physiology·Christy-Lynn M Cooke, Sandra T Davidge
Feb 28, 2002·The Journal of Pharmacology and Experimental Therapeutics·Akiko TanakaKoji Takeuchi
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jon L FreelsRichard A Robbins
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeffrey C HoytRichard A Robbins
Apr 24, 2002·Pulmonary Pharmacology & Therapeutics·Seitaro OkamuraIsamu Miyamori
Aug 13, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ekambaram Padmini, Munuswamy Usha Rani
Oct 15, 2008·Placenta·R P WebsterL Myatt

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