Peroxynitrite-mediated vasorelaxation: evidence against the formation of circulating S-nitrosothiols

The American Journal of Physiology
J E GravesNeil W Kooy

Abstract

Peroxynitrite-induced relaxation of isolated vessels may involve the formation of S-nitrosothiols. This study characterized the hemodynamic effects of systemically injected peroxynitrite in penotobarbital sodium-anesthetized rats and determined whether these effects were due to the formation of S-nitrosothiols. We utilized L-penicillamine, which attenuates the hemodynamic effects of systemically injected S-nitrosothiols. The hemodynamic effects of peroxynitrite and the S-nitrosothiols L-S-nitrosocysteine, L-S-nitrosoglutathione, and S-nitrosoalbumin were determined before and 25 min after the administration of L-penicillamine or saline. Peroxynitrite and the S-nitrosothiols produced dose-dependent reductions in mean arterial pressure and mesenteric and hindquarter vascular resistances. The hypotensive and vasodilator effects of the S-nitrosothiols were significantly reduced by L-penicillamine. In contrast, the hemodynamic actions of peroxynitrite were unaffected by L-penicillamine. Therefore, peroxynitrite produces hypotensive and vasodilator responses in anesthetized rats that are unlikely to be due to the formation of circulating S-nitrosothiols. The mechanisms by which peroxynitrite produces vasodilatation in vivo remain to ...Continue Reading

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Citations

Feb 1, 2006·Clinical and Experimental Pharmacology & Physiology·Jonathan E GravesStephen J Lewis
Jan 13, 2004·Journal of Applied Physiology·B D NossamanP J Kadowitz
Feb 22, 2001·Journal of Applied Physiology·H Y Shin, S C George
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Sep 18, 2020·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Boran KatunaricJulie K Freed

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