PMID: 15223784Jun 30, 2004Paper

Peroxynitrite reaction with eye lens proteins: alpha-crystallin retains its activity despite modification

Investigative Ophthalmology & Visual Science
Geetha ThiagarajanDorairajan Balasubramanian

Abstract

. Peroxynitrite is a highly potent reactive oxygen/nitrogen species present in the environment and also endogenously in the eye, that causes a variety of disorders. This study was undertaken to look at the oxidative damage that peroxynitrite causes to the proteins of the lens and the functional consequences thereof. . Peroxynitrite was allowed to react with alpha-, beta-, and gamma-crystallins. The formation of nitrotyrosine and nitrotryptophan, dityrosine, protein covalent cross-links, and chain degradation products were monitored by spectroscopy and SDS-PAGE. Conformational changes occurring in the protein were monitored with circular dichroism spectroscopy. The chaperoning ability of alpha-crystallin was assayed by monitoring its ability to inhibit the self-aggregation of two test proteins: beta-crystallin and insulin. . Peroxynitrite reaction produced nitrotyrosine, nitrotryptophan, and dityrosine, nondisulfide covalent cross-linked aggregates, and peptide chain degradation. The hydroxyl radicals produced by peroxynitrite caused more chain degradation than did the carbonate radicals. The oxidative reaction caused increased conformational disorder. The yield was highest in gamma-crystallin and least in alpha-crystallin. The ...Continue Reading

Citations

Oct 27, 2009·The Journal of Biological Chemistry·Xingjun FanVincent M Monnier
Aug 28, 2009·Photochemistry and Photobiology·Jolon M DyerKeith R Millington
Sep 16, 2014·Neural Regeneration Research·Yuan LiuShilei Gong
Jun 28, 2016·Journal of Biomolecular Structure & Dynamics·Zarina ArifJamal Ahmad
Apr 15, 2006·Photochemistry and Photobiology·Jolon M DyerWarren G Bryson
Dec 7, 2005·Progress in Retinal and Eye Research·Alice BanhJacob G Sivak

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