PMID: 9525658Apr 3, 1998Paper

Persistence and expression of the herpes simplex virus genome in the absence of immediate-early proteins

Journal of Virology
L A SamaniegoN A DeLuca

Abstract

The immediate-early (IE) proteins of herpes simplex virus (HSV) function on input genomes and affect many aspects of host cell metabolism to ensure the efficient expression and regulation of the remainder of the genome and, subsequently, the production of progeny virions. Due to the many and varied effects of IE proteins on host cell metabolism, their expression is not conducive to normal cell function and viability. This presents a major impediment to the use of HSV as a vector system. In this study, we describe a series of ICP4 mutants that are defective in different subsets of the remaining IE genes. One mutant, d109, does not express any of the IE proteins and carries a green fluorescent protein (GFP) transgene under the control of the human cytomegalovirus IE promoter (HCMVIEp). d109 was nontoxic to Vero and human embryonic lung (HEL) cells at all multiplicities of infection tested and was capable of establishing persistent infections in both of these cell types. Paradoxically, the genetic manipulations that were required to eliminate toxicity and allow the genome to persist in cells for long periods of time also dramatically lowered the level of transgene expression. Efficient expression of the HCMVIEp-GFP transgene in th...Continue Reading

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R W Honess, B Roizman
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R ManservigiA Buchan
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P GhazalR M Evans
May 1, 1992·Journal of Virology·P A JohnsonT Friedmann
Dec 1, 1987·The Journal of General Virology·J RussellC M Preston
Mar 1, 1988·Journal of Virology·A D KwongN Frenkel
May 1, 1985·Molecular and Cellular Biology·M P Quinlan, D M Knipe
Sep 1, 1985·Journal of Virology·W R SacksP A Schaffer
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I H Gelman, S Silverstein
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T M Kristie, B Roizman
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A D Kwong, N Frenkel

❮ Previous
Next ❯

Citations

Jan 21, 2000·The Journal of General Virology·C M Preston
Feb 16, 2000·Gene Therapy·D J FinkJ C Glorioso
Jan 3, 2001·Journal of Virology·K L MossmanJ R Smiley
Oct 3, 2002·Annual Review of Pharmacology and Toxicology·Neeltje A Kootstra, Inder M Verma
Dec 24, 2003·International Reviews of Immunology·Margot L GoodkinJohn A Blaho
Jul 29, 2004·Journal of Virology·Gregory T MelroeDavid M Knipe
Jun 15, 2004·Journal of Virology·Patrick LomonteAlberto L Epstein
Jun 17, 2005·The Journal of General Virology·V GeiserC Jones
Jun 7, 2005·Expert Opinion on Biological Therapy·Luisa BarzonGiorgio Palù
Feb 9, 2011·Immunology and Cell Biology·Daniëlle HorstEmmanuel J H J Wiertz
Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Megan H OrzalliDavid M Knipe
Nov 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Megan H OrzalliDavid M Knipe
Aug 5, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R D Everett
Jun 7, 2002·Journal of Virology·Dool-Bboon Kim, Neal A DeLuca
Oct 12, 2000·Journal of Virology·R D Everett
Nov 10, 2001·Annual Review of Genomics and Human Genetics·A Pfeifer, I M Verma
Apr 27, 2005·The Journal of Gene Medicine·Delphine CuchetAlberto L Epstein
May 3, 2003·Nature Reviews. Genetics·Clare E ThomasMark A Kay

❮ Previous
Next ❯

Related Concepts

Related Feeds

Absence Epilepsy

Absence epilepsy is a common seizure disorder in children which can produce chronic psychosocial sequelae. Discover the latest research on absence epilepsies here.