Persistence of a pKPN3-like CTX-M-15-encoding IncFIIK plasmid in a Klebsiella pneumonia ST17 host during two years of intestinal colonization

PloS One
Iren Høyland LöhrUmaer Naseer

Abstract

To characterize the CTX-M-15-encoding plasmid in a Klebsiella pneumoniae ST17 strain, responsible for an outbreak at a Norwegian neonatal intensive care unit and subsequent colonization of affected children for up to two years. To identify plasmid-mediated features relevant for the outbreak dynamics, and to investigate the plasmids capability of horizontal transfer, its segregational stability and plasmid-mediated fitness costs. Plasmid profiling was performed by S1-nuclease PFGE, PCR-based replicon typing and Southern blot-hybridization. The complete sequence of the CTX-M-15-encoding plasmid was obtained by 454 sequencing. Plasmid self-transferability was investigated by broth- and filter mating, segregational stability was explored by serial passage, and plasmid-conferred fitness costs were examined in pairwise head-to-head competitions and by growth rate comparisons. CTX-M-15 was encoded by a ~180 kb IncFIIK plasmid in K. pneumoniae ST17. S1-nuclease PFGE profiles of the first and the last CTX-M-15-producing K. pneumoniae isolates, recovered from the four children colonized the longest, suggested that the plasmid was stably maintained during intestinal carriage of up to two years. The DNA sequence of the pKPN3-like plasmid, ...Continue Reading

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Citations

Mar 15, 2016·Current Opinion in Microbiology·Changhan LeeUte Römling
Jun 6, 2017·Current Opinion in Infectious Diseases·Julia Johnson, Caroline Quach
Apr 18, 2020·The Journal of Antimicrobial Chemotherapy·Andriniaina RakotondrasoaJean-Marc Collard
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Mar 22, 2018·Canadian Journal of Microbiology·Amanda C Carroll, Alex Wong

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Datasets Mentioned

BETA
LM994717
CP000648
CP002474

Methods Mentioned

BETA
454 Genome Sequencer
PCR

Software Mentioned

GrowthRates
Newbler
Artemis
WebAct
ACT

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