Persistence of Ca2+-sensing receptor expression in functionally active, long-term human parathyroid cell cultures

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
M C RoussanneA Bourdeau

Abstract

An original human parathyroid cell culture model from uremic patients with IIo hyperparathyroidism has been developed, with its main feature being long-term functionally active viability up to 5 months, as assessed by persistent responsiveness to changes of extracellular Ca2+ concentrations ([Ca2+]e). In addition to the inhibitory effect of increasing [Ca2+]e, increasing extracellular phosphate exerted a biphasic effect on parathyroid hormone (PTH) secretion. The presence of the Ca2+-sensing receptor (CaR), on which depends the response to [Ca2+]e and its persistence, has been demonstrated in our culture system both by direct detection and by inhibition of its activity. CaR protein was detected by Western blot analysis with a specific anti-CaR antibody. CaR gene transcripts have been identified by reverse transcription-polymerase chain reaction analysis. mRNA (by in situ hybridization) and protein (by immunocytochemistry) expression were detected for both CaR and PTH. Adding a specific anti-CaR antibody to the medium induced a marked reduction of low [Ca2+]e-stimulated PTH release, which decreased to levels equivalent to those obtained in high [Ca2+]e medium. The described long-term functionality could be due to several factors...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M L BrandiG D Aurbach
Dec 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J J BrookmanG N Hendy
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M L BrandiG D Aurbach
Feb 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R D RidgewayR R MacGregor
Jun 1, 1983·Molecular and Cellular Endocrinology·R R MacGregorD V Cohn
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G N HendyA Rich
Jul 26, 1995·Biochemical and Biophysical Research Communications·A J BrownE Slatopolsky
May 26, 1995·The Journal of Biological Chemistry·J E GarrettF Fuller
Jul 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·F SunL A Fitzpatrick
Feb 1, 1994·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·E Slatopolsky, J A Delmez
Apr 15, 1993·Nature
Apr 1, 1993·Journal of Endocrinological Investigation·M L Brandi
Apr 1, 1996·The Journal of Clinical Endocrinology and Metabolism·O KiforE M Brown
Jul 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y AlmadenM Rodriguez
Sep 1, 1996·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·P K NielsenK Olgaard

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Citations

Dec 19, 2009·World Journal of Surgery·Giuseppe CavallaroGiorgio De Toma
Jan 18, 2006·Annual Review of Medicine·Steven C Hebert
Mar 1, 2010·Expert Opinion on Drug Discovery·Natalia Carrillo-LópezJorge B Cannata-Andía
Nov 10, 2010·Expert Opinion on Biological Therapy·Hirotaka KomabaMasafumi Fukagawa
Nov 28, 2008·Kidney International·Genta KanaiAkira Saito
Feb 17, 2009·Kidney International·Mariano RodríguezYolanda Almadén
Mar 6, 2004·American Journal of Hypertension·Sharla K Sutherland, Christina G Benishin
Mar 31, 2009·Endocrinology and Metabolism Clinics of North America·Edward M Brown
Feb 7, 2015·Pathology, Research and Practice·Jean GogusevFrancis Jaubert
Jul 17, 2001·European Journal of Clinical Investigation·M C RoussanneA Bourdeau
Mar 26, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Cynthia S RitterAlex J Brown
Apr 27, 2019·Biopreservation and Biobanking·Annalisa AitiMarina Buzzi
Jun 20, 2003·Current Opinion in Nephrology and Hypertension·L Darryl Quarles
Jan 12, 2001·Physiological Reviews·E M Brown, R J MacLeod
Aug 31, 2000·Journal of the American Society of Nephrology : JASN·Yolanda AlmadénMariano Rodríguez
Mar 13, 2003·Kidney International·Antonio CanalejoMariano Rodríguez

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