Jan 1, 1985

Persistence of DNA lesions and the cytological cancellation of sister chromatid exchanges

Chromosoma
J B SchvartzmanS Ramos

Abstract

The ability of UV light, mitomycin C and ionizing radiation to induce the formation of sister chromatid exchanges (SCEs) at the same locus in successive cell generations was investigated in human lymphocytes. Cells were exposed to the DNA damaging agents after they had completed their first round of DNA replication, and SCEs were examined at the third division in chromosomes that had been differentially stained three ways. Although some of these treatments induced long-lived lesions that increased the frequency of SCEs in successive cell generations, none of the lesions led to the formation of consecutive SCEs at the same locus in successive cell generations. This observation seriously challenges the hypothesis that SCE cancellation results as a consequence of persistence of the lesions induced by these agents.

Mentioned in this Paper

Mutamycin
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Sister Chromatid Exchange
Lymphoid Cells
Chromosomes, Human
Antibiotics, Cytotoxic
Chromosomes
DNA Replication
Locus
Mitomycin

About this Paper

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