Persistence of F-Specific RNA Coliphages in Surface Waters from a Produce Production Region along the Central Coast of California

PloS One
Subbarao V Ravva, Chester Z Sarreal

Abstract

F+ RNA coliphages (FRNA) are used to source-track fecal contamination and as surrogates for enteric pathogen persistence in the environment. However, the environmental persistence of FRNA is not clearly understood and necessitates the evaluation of the survival of prototype and environmental isolates of FRNA representing all four genogroups in surface waters from the central coast of California. Water temperature played a significant role in persistence-all prototype and environmental strains survived significantly longer at 10°C compared to 25°C. Similarly, the availability of host bacterium was found to be critical in FRNA survival. In the absence of E. coli Famp, all prototypes of FRNA disappeared rapidly with a D-value (days for one log reduction) of <1.2 d from water samples incubated at 25°C; the longest surviving prototype was SP. However, in the presence of the host, the order of persistence at 25°C was QB>MS2>SP>GA and at 10°C it was QB = MS2>GA>SP. Significant differences in survival were observed between prototypes and environmental isolates of FRNA. While most environmental isolates disappeared rapidly at 25°C and in the absence of the host, members of genogroups GIII and GI persisted longer with the host compared t...Continue Reading

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Citations

Mar 18, 2017·Letters in Applied Microbiology·B R McMinnA Korajkic
Oct 4, 2019·Microbiology and Molecular Biology Reviews : MMBR·Asja KorajkicValerie J Harwood
Nov 6, 2020·Journal of Environmental Quality·Ammar SafaieMantha S Phanikumar
Sep 22, 2018·The Science of the Total Environment·Viviane GirardiFernando R Spilki

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Methods Mentioned

BETA
environmental stresses

Software Mentioned

Systat
SigmaPlot

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