Persistence of genetic damage in lymphocytes from former uranium miners

Cytogenetic and Genome Research
Friedo ZölzerJiří Škopek

Abstract

Lymphocytes from former uranium miners who finished work underground one or more decades ago were analysed with respect to possibly persisting genetic damage induced by their radiation exposure. A modified micronucleus-centromere test was used which determined the frequency of micronucleus-containing binucleate cells after cytochalasin B treatment and the percentage of centromere-free micronuclei, assessed with the help of immunofluorescence labeling of centromere protein B. Whereas the overall frequency of micronucleus-containing cells was not significantly elevated above the level found in a control group, former miners showed a greater percentage of centromere-free micronuclei, i.e. micronuclei containing only acentric fragments. Our results are in excellent agreement with those of an earlier uranium miner study and lend support to the assumption that genetic damage from alpha radiation can persist for many years after exposure, possibly due to genomic instability. The frequency of micronucleus-containing cells, but not the percentage of centromere-free micronuclei, significantly increased with time since last exposure in the mines. This can be attributed, at least in part, to the fact that miners who have finished working u...Continue Reading

Citations

Jan 4, 2017·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Friedo ZölzerRadim Šrám
May 31, 2019·International Journal of Radiation Biology·C Meenakshi, B Venkatraman
May 18, 2016·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Olivier LaurentDominique Laurier
Mar 24, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sherin John JosephMohamed Saiyad Musthafa

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