Persistence of Salmonella Typhimurium LT2 in Soil Enhanced after Growth in Lettuce Medium

Frontiers in Microbiology
Eva FornefeldKornelia Smalla

Abstract

The persistence of Salmonella in the environment is influenced by a multitude of biotic and abiotic factors. In addition, its persistence can be influenced by preadaptation before the introduction into the environment. In order to study how preadaptation changes the survival of Salmonella in soil and therefore its potential to colonize the phytosphere, we developed a new medium based on lettuce material [lettuce medium (LM)]. Salmonella enterica serovar Typhimurium strain LT2 was used as a model for Salmonella in this study. LT2 was inoculated into soil microcosms after pregrowth in Luria Bertani (LB) broth or in LM. Survival of LT2 in soil was monitored over 56 days by plate counts and quantification of the Typhimurium-specific gene STM4497 using qPCR in total community DNA for which primers and TaqMan probe were designed in this study. Significantly enhanced persistence was observed for LT2 pregrown in LM compared to LT2 pregrown in LB, indicating a preadaptation effect. Surprisingly, no improved survival could be observed for S. Typhimurium strain 14028s and S. enterica serovar Senftenberg after pregrowth on LM. This indicates a high strain specificity of preadaptation. Results from previous studies suggested that biofilm fo...Continue Reading

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Citations

Dec 20, 2018·Molecular Plant-microbe Interactions : MPMI·Seul I Kim, Hyunjin Yoon
Jan 26, 2020·FEMS Microbiology Letters·Jasper SchierstaedtAdam Schikora
Mar 5, 2020·Environmental Microbiology·Jasper SchierstaedtAdam Schikora
Dec 23, 2017·Frontiers in Microbiology·Julia Aguilera-HerceFrancisco Ramos-Morales
May 7, 2019·Frontiers in Microbiology·Yulia KroupitskiShlomo Sela Saldinger
Oct 8, 2019·FEMS Microbiology Ecology·Azhar A ZarkaniAdam Schikora
Jun 14, 2021·Food Microbiology·Azhar A Zarkani, Adam Schikora

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Methods Mentioned

BETA
PCR
environmental stresses

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