Persistent Activation of cGMP-Dependent Protein Kinase by a Nitrated Cyclic Nucleotide via Site Specific Protein S-Guanylation

Biochemistry
Soichiro AkashiTakaaki Akaike

Abstract

8-Nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP) is a nitrated derivative of guanosine 3',5'-cyclic monophosphate (cGMP) formed endogenously under conditions associated with production of both reactive oxygen species and nitric oxide. It acts as an electrophilic second messenger in the regulation of cellular signaling by inducing a post-translational modification of redox-sensitive protein thiols via covalent adduction of cGMP moieties to protein thiols (protein S-guanylation). Here, we demonstrate that 8-nitro-cGMP potentially S-guanylates thiol groups of cGMP-dependent protein kinase (PKG), the enzyme that serves as one of the major receptor proteins for intracellular cGMP and controls a variety of cellular responses. S-Guanylation of PKG was found to occur in a site specific manner; Cys42 and Cys195 were the susceptible residues among 11 Cys residues. Importantly, S-guanylation at Cys195, which is located in the high-affinity cGMP binding domain of PKG, causes persistent enzyme activation as determined by in vitro kinase assay as well as by an organ bath assay. In vivo, S-guanylation of PKG was demonstrated to occur in mice without any specific treatment and was significantly enhanced by lipopolysaccharide administ...Continue Reading

References

Jul 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M C MartínezF J Romero
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Sep 19, 1998·Free Radical Biology & Medicine·G L Squadrito, W A Pryor
Jun 6, 2003·Journal of Biochemistry and Molecular Biology·Euikyung Kim, Ji Man Park
Jun 25, 2004·Journal of Chromatography. a·A Berthod, S Carda-Broch
Apr 27, 2007·Vascular Pharmacology·Pei-Han XuZhong-Li Liu
Aug 25, 2007·Science·Joseph R BurgoynePhilip Eaton
Sep 13, 2007·Nature Reviews. Molecular Cell Biology·Benoît D'Autréaux, Michel B Toledano
Oct 2, 2007·Nature Chemical Biology·Tomohiro SawaTakaaki Akaike
Dec 18, 2007·Journal of Molecular Biology·Vera AlverdiAlbert J R Heck
Aug 8, 2008·American Journal of Physiology. Cell Physiology·Dean P Jones
Dec 18, 2008·Handbook of Experimental Pharmacology·Franz HofmannPascal Weinmeister
Jul 6, 2010·Nitric Oxide : Biology and Chemistry·Takaaki AkaikeHideshi Ihara
Aug 19, 2010·Pharmacological Reviews·Sharron H FrancisDavid Sibley
Nov 5, 2010·Diabetes, Obesity & Metabolism·D P Jones, Y-M Go
Jan 15, 2011·British Journal of Pharmacology·Yoshiko TokutomiShokei Kim-Mitsuyama
Sep 21, 2011·Chemical Reviews·Francisco J SchopferBruce A Freeman
Jul 10, 2012·Nature Chemical Biology·Motohiro NishidaTakaaki Akaike
Sep 18, 2012·Antioxidants & Redox Signaling·Md Mizanur RahamanTakaaki Akaike
May 2, 2013·Nitric Oxide : Biology and Chemistry·Tomohiro SawaTakaaki Akaike
Jul 12, 2013·Antioxidants & Redox Signaling·Jose ViñaMari Carmen Gomez-Cabrera
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tomoaki IdaTakaaki Akaike
May 24, 2014·Nature Reviews. Molecular Cell Biology·Kira M Holmström, Toren Finkel
Jun 2, 2015·Trends in Pharmacological Sciences·Yasuko Iwakiri, Moon Young Kim

❮ Previous
Next ❯

Citations

Mar 26, 2016·Journal of Clinical Biochemistry and Nutrition·Motohiro NishidaTakaaki Akaike
Jun 15, 2016·Cardiovascular Research·Peter P Rainer, David A Kass
Jul 31, 2016·Biochemical and Biophysical Research Communications·Hiroyasu TsutsukiTomohiro Sawa
Dec 17, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shingo KasamatsuTakaaki Akaike
Jun 17, 2020·Antioxidants & Redox Signaling·Shingo Kasamatsu
Jun 9, 2020·Frontiers in Plant Science·Marek Petřivalský, Lenka Luhová
Sep 1, 2017·Scientific Reports·Sonia DonzelliFriederike Cuello
Feb 5, 2021·Journal of Clinical Biochemistry and Nutrition·Tianli ZhangTomohiro Sawa
Aug 28, 2018·Chemical Reviews·Saba ParvezYimon Aye
Aug 25, 2017·Chemical Research in Toxicology·Hideshi IharaTakaaki Akaike

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.