PMID: 8972393Dec 1, 1996Paper

Persistent activation of transducin by bleached rhodopsin in salamander rods

The Journal of General Physiology
H R MatthewsG L Fain

Abstract

The hydrolysis-resistant GTP analogue GTP-gamma-S was introduced into rods isolated from the retina of the salamander Ambystoma tigrinum to study the origin of the persistent excitation induced by intense bleaching illumination. Dialysis of a dark-adapted rod with a whole-cell patch pipette containing 2 mM GTP-gamma-S resulted in a gradual decrease in circulating current. If the rod was first bleached and its sensitivity allowed to stabilize for at least 30 min, then dialysis with GTP-gamma-S produced a much faster current decay. The circulating current could be restored by superfusion with the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, suggesting that the decay in current originated from persistent excitation of the phosphodiesterase by transducin bound to GTP-gamma-S. We conclude that the persistent excitation which follows bleaching is likely to involve the GTP-binding protein transducin, which mediates the normal photoresponse. This observation suggests that a form of rhodopsin which persists long after bleaching can activate transducin much as does photoisomerized rhodopsin, although with considerably lower gain.

Citations

Aug 1, 1997·Current Opinion in Neurobiology·K Palczewski, J C Saari
Nov 9, 2000·Progress in Retinal and Eye Research·T Ebrey, Y Koutalos
Jun 8, 2001·Progress in Retinal and Eye Research·J K McBeeD R Pepperberg
Feb 14, 1998·The Journal of General Physiology·A P SampathG L Fain
Mar 29, 2006·The Journal of General Physiology·Sergei S NikonovEdward N Pugh
Sep 27, 2006·The Journal of General Physiology·Quanhua HeClint L Makino
Nov 15, 2006·The Journal of General Physiology·Maureen E EstevezM Carter Cornwall
Feb 27, 2009·Behavioral and Brain Functions : BBF·F Josef van der StaayRebecca E Nordquist
Jun 17, 1998·Experimental Eye Research·A Surya, B E Knox
Mar 21, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Gordon L Fain
Jan 28, 2020·The Journal of General Physiology·Norianne T IngramGordon L Fain
Jan 12, 2001·Physiological Reviews·G L FainY Koutalos
Nov 27, 2019·Investigative Ophthalmology & Visual Science·Ryo KubotaRobert A Linsenmeier
Aug 28, 2020·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ryo KubotaJeffrey K Gregory

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