Persistent and transient antibody responses to hepatitis E virus detected by western immunoblot using open reading frame 2 and 3 and glutathione S-transferase fusion proteins.

Journal of Clinical Microbiology
F LiD A Anderson

Abstract

Recombinant proteins containing amino acid sequences from open reading frame (ORF) 2 and ORF3 of a Chinese strain of hepatitis E virus (HEV) were constructed as fusions with glutathione S-transferase (GST). Stable fusion proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the proteins were transferred to nitrocellulose membranes, and the immobilized proteins were probed with sera from hepatitis E patients from various regions or from rhesus monkeys (Macaca mulatta) experimentally infected with the Chinese strain of HEV. Immunoglobulin G-class antibodies were detected with chemiluminescence and anti-human immunoglobulin G conjugated to horseradish peroxidase. Anti-ORF3 antibodies were detected in most patients and monkeys within 17 days of exposure, but this humoral response declined with time and was usually undetectable by approximately 100 days. Anti-ORF2.1 antibody was usually detected as early as anti-ORF3 but persisted in all animals and many patients, whereas reactivity to the larger GST-ORF2.2 fusion protein was more transient, even though all sequences present in GST-ORF2.1 are present in GST-ORF2.2. Rechallenge of these monkeys with HEV suggested that immunity to reinfection was incomp...Continue Reading

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Citations

Jun 9, 2005·Clinical and Diagnostic Laboratory Immunology·Fan LiCarol A Hartley
Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E E MastR H Purcell
Dec 11, 2002·Journal of Gastroenterology and Hepatology·A ObriadinaY E Khudyakov
Mar 22, 2005·Journal of Medical Virology·Ariel QuintanaDavid Anderson
Mar 17, 2000·Journal of Gastroenterology and Hepatology·R Aggarwal, K Krawczynski
Oct 26, 2005·Chinese Journal of Digestive Diseases·Yan ChenNing Shao Xia
Dec 13, 2003·Journal of Virology·Milan SurjitSunil K Lal
Jan 2, 2009·Journal of Immunoassay & Immunochemistry·T I UlanovaY E Khudyakov
Apr 20, 2001·Journal of Food Protection·J L Smith
Jun 6, 2013·Biomarkers in Medicine·Khayriyyah Mohd HanafiahDavid Anderson
Apr 9, 2019·Transboundary and Emerging Diseases·Javier Caballero-GómezIgnacio García-Bocanegra
Mar 20, 1995·The Medical Journal of Australia·T C HeathD A Anderson
Aug 17, 1999·Journal of Clinical Microbiology·Y E KhudyakovH A Fields
Nov 6, 2009·Virology Journal·Kai ZhaoXueming Tang

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