PMID: 8980668Dec 1, 1996Paper

Persistent increase in the rates of apoptosis and dicentric chromosomes in surviving V79 cells after X-irradiation

International Journal of Radiation Biology
M Jamali, K R Trott

Abstract

In parallel to delayed reproductive death and delayed micronuclei in surviving V79 cells that have been reported, the frequency of apoptosis and dicentrics was investigated in the same cell line, 1-2 weeks after X-ray doses between 1 and 12 Gy. Apoptosis was assessed by morphological criteria after staining with Giemsa and using the Apoptag method in parallel. The frequency of apoptosis peaked 48 h after irradiation at about 35% as a function of dose and then decreased to about 10% on day 3. It remained 10 times higher than in unirradiated cells until day 14. The frequency of dicentric chromosomes 24 h after irradiation increased according to a linear-quadratic function, up to about 110% after 12 Gy, decreased 3 days later to about 10% and remained about 5-fold higher than in control cells. The dose-response relationship was similar for all the various end-points of expression of radiation induced genomic instability, showing a steep increase of the frequency with dose up to 3-4 Gy and with no further increase at higher radiation doses.

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Sep 1, 1991·International Journal of Radiation Biology·W P Chang, J B Little
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Mar 1, 1996·International Journal of Radiation Biology·M Jamali, K R Trott

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Citations

Dec 25, 2010·Radiation Protection Dosimetry·S G Andreev, Y A Eidelman
Jan 9, 1999·International Journal of Radiation Biology·K R TrottA Teibe
Jul 26, 2011·Mutation Research·Yaroslav Ilnytskyy, Olga Kovalchuk
Sep 15, 2010·Environmental and Molecular Mutagenesis·Stephano S MelloElza T Sakamoto-Hojo
May 7, 2014·Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis·Katriina HuumonenJukka Juutilainen
Jul 24, 2008·Dose-response : a Publication of International Hormesis Society·Helmut Schöllnberger, Peter M Eckl
Jun 5, 1999·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·D BartkowiakE M Röttinger
Aug 27, 2013·Doklady. Biochemistry and Biophysics·S G AndreevS V Slanina
Oct 19, 2000·Journal of Radiation Research·M AokiT Yamada
Nov 2, 2007·Radiation Research·H SchöllnbergerW Hofmann
Sep 22, 2001·Cancer Genetics and Cytogenetics·J L SchwartzH H Evans

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis